PDB entry 3e2w

View 3e2w on RCSB PDB site
Description: H. influenzae beta-carbonic anhydrase, variant Y181F with 1M bicarbonate
Class: lyase
Keywords: beta carbonic anhydrase, allosteric site mutant, Lyase, Metal-binding
Deposited on 2008-08-06, released 2009-08-18
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.207
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2wa_
  • Chain 'B':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2wb_
  • Chain 'C':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2wc_
  • Chain 'D':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2wd_
  • Chain 'E':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2we_
  • Chain 'F':
    Compound: Carbonic anhydrase 2
    Species: Haemophilus influenzae [TaxId:727]
    Gene: can, HI1301
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45148 (0-End)
      • engineered (180)
    Domains in SCOPe 2.04: d3e2wf_
  • Heterogens: ZN, SO4, BCT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3e2wA (A:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wA (A:)
mdkikqlfannyswaqrmkeenqtphylwigcsdsrvpaekltnlepgelfvhrnvanqv
ihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkh
ghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvd
qgvmatsretleisyrnaiarlsildee

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3e2wB (B:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wB (B:)
mdkikqlfannyswaqrtphylwigcsdsrvpaekltnlepgelfvhrnvanqvihtdfn
clsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkhghllgk
lspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvdqgvmat
sretleisyrnaiarlsilde

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3e2wC (C:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wC (C:)
mdkikqlfannyswaqrmkqtphylwigcsdsrvpaekltnlepgelfvhrnvanqviht
dfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkhghl
lgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvdqgv
matsretleisyrnaiarlsild

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3e2wD (D:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wD (D:)
mdkikqlfannyswaqrmkeenqtphylwigcsdsrvpaekltnlepgelfvhrnvanqv
ihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkh
ghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvd
qgvmatsretleisyrnaiarlsi

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3e2wE (E:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wE (E:)
mdkikqlfannyswaqrmqtphylwigcsdsrvpaekltnlepgelfvhrnvanqvihtd
fnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkhghll
gklspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvdqgvm
atsretleisyrnaiarlsild

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3e2wF (F:)
mdkikqlfannyswaqrmkeenstyfkeladhqtphylwigcsdsrvpaekltnlepgel
fvhrnvanqvihtdfnclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwl
lhirdiwfkhghllgklspekradmltkinvaeqvynlgrtsivksawergqklslhgwv
fdvndgflvdqgvmatsretleisyrnaiarlsildeenilkkdhlent

    Sequence, based on observed residues (ATOM records): (download) 
    >3e2wF (F:)
mdkikqlfannyswaqrmtphylwigcsdsrvpaekltnlepgelfvhrnvanqvihtdf
nclsvvqyavdvlkiehiiicghtncggihaamadkdlglinnwllhirdiwfkhghllg
klspekradmltkinvaeqvynlgrtsivksawergqklslhgwvfdvndgflvdqgvma
tsretleisyrnaiarlsi