PDB entry 2wnq

View 2wnq on RCSB PDB site
Description: structure of the e192n mutant of e. coli n-acetylneuraminic acid lyase in space group p21
Class: lyase
Keywords: substrate specificity, carbohydrate metabolism, directed evolution, protein engineering, lyase, aldolase
Deposited on 2009-07-17, released 2010-08-25
The last revision prior to the SCOPe 2.04 freeze date was dated 2010-11-17, with a file datestamp of 2010-11-12.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.19928
AEROSPACI score: 0.49 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.04: d2wnqa_
  • Chain 'B':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4
      • engineered mutation (198)
    Domains in SCOPe 2.04: d2wnqb_
  • Chain 'C':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • expression tag (7)
      • engineered mutation (198)
    Domains in SCOPe 2.04: d2wnqc_
  • Chain 'D':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.04: d2wnqd_
  • Heterogens: CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wnqA (A:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnqA (A:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wnqB (B:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnqB (B:)
tnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqv
leivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhy
raiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehp
dlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkv
idlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2wnqC (C:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnqC (C:)
hatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsere
qvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcd
hyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirre
hpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecn
kvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2wnqD (D:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnqD (D:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqe