PDB entry 2wkt

View 2wkt on RCSB PDB site
Description: biosynthetic thiolase from z. ramigera. complex of the n316a mutant with coenzyme a.
Class: transferase
Keywords: acyltransferase, phb biosynthesis, transferase, thiolase fold
Deposited on 2009-06-18, released 2009-11-03
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-11-02, with a file datestamp of 2011-10-28.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.1891
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKT (10-10)
    Domains in SCOPe 2.04: d2wkta1, d2wkta2
  • Chain 'B':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKT (10-10)
    Domains in SCOPe 2.04: d2wktb1, d2wktb2
  • Chain 'C':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKT (10-10)
    Domains in SCOPe 2.04: d2wktc1, d2wktc2
  • Chain 'D':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKT (10-10)
    Domains in SCOPe 2.04: d2wktd1, d2wktd2
  • Heterogens: SO4, K, CL, COA, NA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wktA (A:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wktA (A:)
psiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpage
gqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsma
phcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavas
qnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvt
agnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkaler
agwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntll
femkrrgarkglatlcigggmgvamciesl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wktB (B:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wktB (B:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2wktC (C:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wktC (C:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2wktD (D:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wktD (D:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaaeafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl