PDB entry 2rhr

View 2rhr on RCSB PDB site
Description: P94L actinorhodin ketordeuctase mutant, with NADPH and Inhibitor Emodin
Class: oxidoreductase
Keywords: oxidoreductase, polyketide, actinorhodin, ketoreductase, combinatorial biosynthesis, short chain dehydrogenase/reductase
Deposited on 2007-10-09, released 2008-08-19
The last revision prior to the SCOPe 2.02 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.184
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Actinorhodin Polyketide Ketoreductase
    Species: STREPTOMYCES COELICOLOR
    Gene: actIII
    Database cross-references and differences (RAF-indexed):
    • Uniprot P16544 (Start-276)
      • engineered (109)
    Domains in SCOPe 2.02: d2rhra_
  • Chain 'B':
    Compound: Actinorhodin Polyketide Ketoreductase
    Species: STREPTOMYCES COELICOLOR
    Gene: actIII
    Database cross-references and differences (RAF-indexed):
    • Uniprot P16544 (16-276)
      • expression tag (13-15)
      • engineered (109)
    Domains in SCOPe 2.02: d2rhrb_
  • Heterogens: NAP, EMO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2rhrA (A:)
hhhhhhssglvprgshmatqdsevalvtgatsgigleiarrlgkeglrvfvcargeeglr
ttlkelreagveadgrtcdvrsvpeiealvaavverygpvdvlvnnagrlgggataelad
elwldvvetnltgvfrvtkqvlkaggmlergtgrivniastggkqgvvhaapysaskhgv
vgftkalglelartgitvnavcpgfvetpmaasvrehysdiwevsteeafdritarvpig
ryvqpsevaemvayligpgaaavtaqalnvcgglgny

    Sequence, based on observed residues (ATOM records): (download) 
    >2rhrA (A:)
sevalvtgatsgigleiarrlgkeglrvfvcargeeglrttlkelreagveadgrtcdvr
svpeiealvaavverygpvdvlvnnagrlgggataeladelwldvvetnltgvfrvtkqv
lkaggmlergtgrivniastggkqgvvhaapysaskhgvvgftkalglelartgitvnav
cpgfvetpmaasvrehysdiwevsteeafdritarvpigryvqpsevaemvayligpgaa
avtaqalnvcgglgny

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2rhrB (B:)
hhhhhhssglvprgshmatqdsevalvtgatsgigleiarrlgkeglrvfvcargeeglr
ttlkelreagveadgrtcdvrsvpeiealvaavverygpvdvlvnnagrlgggataelad
elwldvvetnltgvfrvtkqvlkaggmlergtgrivniastggkqgvvhaapysaskhgv
vgftkalglelartgitvnavcpgfvetpmaasvrehysdiwevsteeafdritarvpig
ryvqpsevaemvayligpgaaavtaqalnvcgglgny

    Sequence, based on observed residues (ATOM records): (download) 
    >2rhrB (B:)
gshmatqdsevalvtgatsgigleiarrlgkeglrvfvcargeeglrttlkelreagvea
dgrtcdvrsvpeiealvaavverygpvdvlvnnagrlgggataeladelwldvvetnltg
vfrvtkqvlkaggmlergtgrivniastggkqgvvhaapysaskhgvvgftkalglelar
tgitvnavcpgfvetpmaasvrehteeafdritarvpigryvqpsevaemvayligpgaa
avtaqalnvcgglgny