PDB entry 2oxv

View 2oxv on RCSB PDB site
Description: Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.
Class: hydrolase/DNA
Keywords: EcoRI, type II restriction endonuclease, protein-DNA interactions, promiscuous mutant, relaxed specificity mutant, hydrolase/DNA COMPLEX
Deposited on 2007-02-21, released 2007-10-23
The last revision prior to the SCOPe 2.04 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: 0.202
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Type II restriction enzyme EcoRI
    Species: Escherichia coli [TaxId:562]
    Gene: ecoRIR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00642 (Start-276)
      • conflict (137)
    Domains in SCOPe 2.04: d2oxva_
  • Chain 'C':
    Compound: DNA (5'-d(*tp*cp*gp*cp*gp*ap*ap*tp*tp*cp*gp*cp*g)-3')
    Species: synthetic, synthetic
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2oxvA (A:)
msnkkqsnrlteqhklsqgvigifgdyakahdlavgevsklvkkalsneypqlsfryrds
ikkteinealkkidpdlggtlfvsnssikpdggivevkddygewrvvlvaeakhqgkdii
nirngllvgkrgdqdlmtagnaiershkniseianfmlseshfpyvlflegsnfltenis
itrpdgrvvnleynsgilnrldrltaanygmpinsnlcinkfvnhkdksimlqaasiytq
gdgrewdskimfeimfdisttslrvlgrdlfeqltsk

    Sequence, based on observed residues (ATOM records): (download) 
    >2oxvA (A:)
sqgvigifgdyakahdlavgevsklvkkalsneypqlsfryrdsikkteinealkkidpd
lggtlfvsnssikpdggivevkddygewrvvlvaeakhqgkdiinirngllvgkrgdqdl
mtagnaiershkniseianfmlseshfpyvlflegsnfltenisitrpdgrvvnleynsg
ilnrldrltaanygmpinsnlcinkfvnhkdksimlqaasiytqgdgrewdskimfeimf
disttslrvlgrdlfeqltsk

  • Chain 'C':
    No sequence available.