PDB entry 2ofj

View 2ofj on RCSB PDB site
Description: Crystal structure of the E190A mutant of o-succinylbenzoate synthase from Escherichia coli
Class: lyase
Keywords: TIM barrel, LYASE
Deposited on 2007-01-03, released 2007-06-12
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.225
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: o-succinylbenzoate synthase
    Species: Escherichia coli [TaxId:511145]
    Gene: menC, b2261, JW2256
    Database cross-references and differences (RAF-indexed):
    • Uniprot P29208 (3-322)
      • engineered (192)
    Domains in SCOPe 2.08: d2ofja1, d2ofja2
  • Chain 'B':
    Compound: o-succinylbenzoate synthase
    Species: Escherichia coli [TaxId:511145]
    Gene: menC, b2261, JW2256
    Database cross-references and differences (RAF-indexed):
    • Uniprot P29208 (3-322)
      • engineered (192)
    Domains in SCOPe 2.08: d2ofjb1, d2ofjb2
  • Chain 'C':
    Compound: o-succinylbenzoate synthase
    Species: Escherichia coli [TaxId:511145]
    Gene: menC, b2261, JW2256
    Database cross-references and differences (RAF-indexed):
    • Uniprot P29208 (3-322)
      • engineered (192)
    Domains in SCOPe 2.08: d2ofjc1, d2ofjc2
  • Chain 'D':
    Compound: o-succinylbenzoate synthase
    Species: Escherichia coli [TaxId:511145]
    Gene: menC, b2261, JW2256
    Database cross-references and differences (RAF-indexed):
    • Uniprot P29208 (3-322)
      • engineered (192)
    Domains in SCOPe 2.08: d2ofjd1, d2ofjd2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2ofjA (A:)
gsamrsaqvyrwqipmdagvvlrdrrlktrdglyvclregeregwgeisplpgfsqetwe
eaqsvllawvnnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcngdpddl
ilkladmpgekvakvkvglyeavrdgmvvnllleaipdlhlrldanrawtplkgqqfaky
vnpdyrdriaflaepcktrddsrafaretgiaiawdeslrepdfafvaeegvravvikpt
ltgslekvreqvqaahalgltavisssiesslgltqlariaawltpdtipgldtldlmqa
qqvrrwpgstlpvvevdalerll

    Sequence, based on observed residues (ATOM records): (download) 
    >2ofjA (A:)
mrsaqvyrwqipmdrrlktrdglyvclregeregwgeisplpgfsqetweeaqsvllawv
nnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcnpgekvakvkvglyeav
rdgmvvnllleaipdlhlrldanrawtplkgqqfakyvnpdyrdriaflaepcktrddsr
afaretgiaiawdeslrepdfafvaeegvravvikptltgslekvreqvqaahalgltav
isssiesslgltqlariaawltpdtipgldtldlmqaqqvrrwpgstlpvvevdalerll

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2ofjB (B:)
gsamrsaqvyrwqipmdagvvlrdrrlktrdglyvclregeregwgeisplpgfsqetwe
eaqsvllawvnnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcngdpddl
ilkladmpgekvakvkvglyeavrdgmvvnllleaipdlhlrldanrawtplkgqqfaky
vnpdyrdriaflaepcktrddsrafaretgiaiawdeslrepdfafvaeegvravvikpt
ltgslekvreqvqaahalgltavisssiesslgltqlariaawltpdtipgldtldlmqa
qqvrrwpgstlpvvevdalerll

    Sequence, based on observed residues (ATOM records): (download) 
    >2ofjB (B:)
mrsaqvyrwqipmdrrlktrdglyvclregeregwgeisplpgfsqetweeaqsvllawv
nnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcnpgekvakvkvglyeav
rdgmvvnllleaipdlhlrldanrawtplkgqqfakyvnpdyrdriaflaepcktrddsr
afaretgiaiawdeslrepdfafvaeegvravvikptltgslekvreqvqaahalgltav
isssiesslgltqlariaawltpdtipgldtldlmqaqqvrrwpgstlpvvevdalerll

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2ofjC (C:)
gsamrsaqvyrwqipmdagvvlrdrrlktrdglyvclregeregwgeisplpgfsqetwe
eaqsvllawvnnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcngdpddl
ilkladmpgekvakvkvglyeavrdgmvvnllleaipdlhlrldanrawtplkgqqfaky
vnpdyrdriaflaepcktrddsrafaretgiaiawdeslrepdfafvaeegvravvikpt
ltgslekvreqvqaahalgltavisssiesslgltqlariaawltpdtipgldtldlmqa
qqvrrwpgstlpvvevdalerll

    Sequence, based on observed residues (ATOM records): (download) 
    >2ofjC (C:)
mrsaqvyrwqipmdlktrdglyvclregeregwgeisplpgfsqetweeaqsvllawvnn
wlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcnekvakvkvglyeavrdgm
vvnllleaipdlhlrldanrawtplkgqqfakyvnpdyrdriaflaepcktrddsrafar
etgiaiawdeslrepdfafvaeegvravvikptltgslekvreqvqaahalgltavisss
iesslgltqlariaawltpdtipgldtldlmqaqqvrrwpgstlpvvevdalerll

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2ofjD (D:)
gsamrsaqvyrwqipmdagvvlrdrrlktrdglyvclregeregwgeisplpgfsqetwe
eaqsvllawvnnwlagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcngdpddl
ilkladmpgekvakvkvglyeavrdgmvvnllleaipdlhlrldanrawtplkgqqfaky
vnpdyrdriaflaepcktrddsrafaretgiaiawdeslrepdfafvaeegvravvikpt
ltgslekvreqvqaahalgltavisssiesslgltqlariaawltpdtipgldtldlmqa
qqvrrwpgstlpvvevdalerll

    Sequence, based on observed residues (ATOM records): (download) 
    >2ofjD (D:)
mrsaqvyrwqipmlktrdglyvclregeregwgeisplpgfsqetweeaqsvllawvnnw
lagdcelpqmpsvafgvscalaeltdtlpqaanyraaplcngdpekvakvkvglyeavrd
gmvvnllleaipdlhlrldanrawtplkgqqfakyvnpdyrdriaflaepcktrddsraf
aretgiaiawdeslrepdfafvaeegvravvikptltgslekvreqvqaahalgltavis
ssiesslgltqlariaawltpdtipgldtldlmqaqqvrrwpgstlpvvevdalerll