PDB entry 2nws

View 2nws on RCSB PDB site
Description: Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Class: transferase
Keywords: kdo, kdo8p, kdo8ps, pep, a5p, transferase
Deposited on 2006-11-16, released 2007-04-24
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.234
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Aquifex aeolicus [TaxId:63363]
    Gene: kdsA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O66496
      • engineered (10)
    Domains in SCOPe 2.04: d2nwsa_
  • Chain 'B':
    Compound: 2-dehydro-3-deoxyphosphooctonate aldolase
    Species: Aquifex aeolicus [TaxId:63363]
    Gene: kdsA
    Database cross-references and differences (RAF-indexed):
    • Uniprot O66496
      • engineered (10)
    Domains in SCOPe 2.04: d2nwsb_
  • Heterogens: PEP, A5P, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2nwsA (A:)
mekflviagpnaieseelllkvgeeikrlsekfkevefvfkssfdkanrssihsfrghgl
eygvkalrkvkeefglkittdiheswqaepvaevadiiqipaflcrqtdlllaaaktgra
vnvkkgqflapwdtknvveklkfggakeiyltergttfgynnlvvdfrslpimkqwakvi
ydathsvqlpgglgdksggmrefifpliraavavgcdgvfmethpepekalsdastqlpl
sqlegiieaileirevaskyyetipvk

    Sequence, based on observed residues (ATOM records): (download) 
    >2nwsA (A:)
ekflviagpnaieseelllkvgeeikrlsekfkevefvfkssfdkanrssihsfrghgle
ygvkalrkvkeefglkittdiheswqaepvaevadiiqipaflcrqtdlllaaaktgrav
nvkkgqflapwdtknvveklkfggakeiyltergttfgynnlvvdfrslpimkqwakviy
dathsvqlpgglgdksggmrefifpliraavavgcdgvfmethpepekalsdastqlpls
qlegiieaileirevaskyyeti

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2nwsB (B:)
mekflviagpnaieseelllkvgeeikrlsekfkevefvfkssfdkanrssihsfrghgl
eygvkalrkvkeefglkittdiheswqaepvaevadiiqipaflcrqtdlllaaaktgra
vnvkkgqflapwdtknvveklkfggakeiyltergttfgynnlvvdfrslpimkqwakvi
ydathsvqlpgglgdksggmrefifpliraavavgcdgvfmethpepekalsdastqlpl
sqlegiieaileirevaskyyetipvk

    Sequence, based on observed residues (ATOM records): (download) 
    >2nwsB (B:)
kflviagpnaieseelllkvgeeikrlsekfkevefvfkssfdkanrssihsfrghgley
gvkalrkvkeefglkittdiheswqaepvaevadiiqipaflcrqtdlllaaaktgravn
vkkgqflapwdtknvveklkfggakeiyltergttfgynnlvvdfrslpimkqwakviyd
athsvqlpgglgdksggmrefifpliraavavgcdgvfmethpepekalsdastqlplsq
legiieaileirevaskyyeti