PDB entry 2iwm

View 2iwm on RCSB PDB site
Description: precursor mutant cys1ser of penicillin v acylase from bacillus sphaericus
Class: hydrolase
Keywords: zymogen, hydrolase, precursor, penicillin, autoproteolysis, antibiotic resistance
Deposited on 2006-07-01, released 2007-08-07
The last revision prior to the SCOPe 2.06 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.203
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: penicillin acylase
    Species: BACILLUS SPHAERICUS [TaxId:1421]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12256 (0-End)
      • engineered mutation (3)
      • conflict (100)
    Domains in SCOPe 2.06: d2iwma_
  • Chain 'B':
    Compound: penicillin acylase
    Species: BACILLUS SPHAERICUS [TaxId:1421]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12256 (0-End)
      • engineered mutation (3)
      • conflict (100)
    Domains in SCOPe 2.06: d2iwmb_
  • Chain 'C':
    Compound: penicillin acylase
    Species: BACILLUS SPHAERICUS [TaxId:1421]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12256 (0-End)
      • engineered mutation (3)
      • conflict (100)
    Domains in SCOPe 2.06: d2iwmc_
  • Chain 'D':
    Compound: penicillin acylase
    Species: BACILLUS SPHAERICUS [TaxId:1421]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P12256 (0-End)
      • engineered mutation (3)
      • conflict (100)
    Domains in SCOPe 2.06: d2iwmd_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2iwmA (A:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqvnvms

    Sequence, based on observed residues (ATOM records): (download) 
    >2iwmA (A:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqv

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2iwmB (B:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqvnvms

    Sequence, based on observed residues (ATOM records): (download) 
    >2iwmB (B:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqv

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2iwmC (C:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqvnvms

    Sequence, based on observed residues (ATOM records): (download) 
    >2iwmC (C:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2iwmD (D:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnqvnvms

    Sequence, based on observed residues (ATOM records): (download) 
    >2iwmD (D:)
mlgssslsirttddkslfartmdftmepdskviivprnygirllekenvvinnsyafvgm
gstditspvlydgvnekglmgamlyyatfatyadepkkgtrginpvyvisqvlgncvtvd
dviekltsytllneaniilgfapplhytftdasgesiviepdktgitihrktigvmtnsp
gyewhqtnlrayigvtpnppqdimmgdldltpfgqgagglglpgdftpsarflrvaywkk
ytekaknetegvtnlfhilssvnipkgvvltnegktdytiytsamcaqsknyyfklydns
risavslmaenlnsqdlitfewdrkqdikqlnq