PDB entry 2ibt

View 2ibt on RCSB PDB site
Description: Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position and an abasic site analog at the target base partner position
Class: transferase/DNA
Keywords: DNA, DNA methyltransferase, 2-aminopurine, abasic site analog, base flipping, nucleotide flipping
Deposited on 2006-09-12, released 2007-05-29
The last revision prior to the SCOP 1.73 freeze date was dated 2007-05-29, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.169
AEROSPACI score: 0.56 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: modification methylase taqi
    Species: Thermus aquaticus
    Gene: taqIM
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2ibta1, d2ibta2
  • Chain 'B':
    Compound: 5'-d(*gp*tp*tp*cp*gp*(2pr)p*tp*gp*tp*c)-3'
    Species: synthetic, synthetic
  • Chain 'C':
    Compound: 5'-d(*gp*ap*cp*ap*(3dr)p*cp*gp*(6ma)p*ap*c)-3'
    Species: synthetic, synthetic
  • Chain 'D':
    Compound: modification methylase taqi
    Species: Thermus aquaticus
    Gene: taqIM
    Database cross-references and differences (RAF-indexed): Domains in SCOP 1.73: d2ibtd1, d2ibtd2
  • Chain 'E':
    Compound: 5'-d(*gp*tp*tp*cp*gp*(2pr)p*tp*gp*tp*c)-3'
    Species: synthetic, synthetic
  • Chain 'F':
    Compound: 5'-d(*gp*ap*cp*ap*(3dr)p*cp*gp*(6ma)p*ap*c)-3'
    Species: synthetic, synthetic
  • Heterogens: NEA, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2ibtA (A:)
mglppllslpsnsaprslgrvetppevvdfmvslaeaprggrvlepacahgpflrafrea
hgtayrfvgveidpkaldlppwaegiladfllwepgeafdlilgnppygivgeaskypih
vfkavkdlykkafstwkgkynlygaflekavrllkpggvlvfvvpatwlvledfallref
laregktsvyylgevfpqkkvsavvirfqksgkglslwdtqesesgftpilwaeyphweg
eiirfeteetrkleisgmplgdlfhirfaarspefkkhpavrkepgpglvpvltgrnlkp
gwvdyeknhsglwmpkerakelrdfyatphlvvahtkgtrvvaawderaypwreefhllp
kegvrldpsslvqwlnseamqkhvrtlyrdfvphltlrmlerlpvrreygfhtspesarn
f

    Sequence, based on observed residues (ATOM records): (download) 
    >2ibtA (A:)
vetppevvdfmvslaeaprggrvlepacahgpflrafreahgtayrfvgveidpkaldlp
pwaegiladfllwepgeafdlilgnppygivgeaskypihvfkavkdlykkafstwkgky
nlygaflekavrllkpggvlvfvvpatwlvledfallreflaregktsvyylgevfpqkk
vsavvirfqksgkglslwdtqesesgftpilwaeyphwegeiirfeteetrkleisgmpl
gdlfhirfaarspefkkhpavrkepgpglvpvltgrnlkpgwvdyeknhsglwmpkerak
elrdfyatphlvvahtkgtrvvaawderaypwreefhllpkegvrldpsslvqwlnseam
qkhvrtlyrdfvphltlrmlerlpvrreygfht

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2ibtD (D:)
mglppllslpsnsaprslgrvetppevvdfmvslaeaprggrvlepacahgpflrafrea
hgtayrfvgveidpkaldlppwaegiladfllwepgeafdlilgnppygivgeaskypih
vfkavkdlykkafstwkgkynlygaflekavrllkpggvlvfvvpatwlvledfallref
laregktsvyylgevfpqkkvsavvirfqksgkglslwdtqesesgftpilwaeyphweg
eiirfeteetrkleisgmplgdlfhirfaarspefkkhpavrkepgpglvpvltgrnlkp
gwvdyeknhsglwmpkerakelrdfyatphlvvahtkgtrvvaawderaypwreefhllp
kegvrldpsslvqwlnseamqkhvrtlyrdfvphltlrmlerlpvrreygfhtspesarn
f

    Sequence, based on observed residues (ATOM records): (download) 
    >2ibtD (D:)
vetppevvdfmvslaeaprggrvlepacahgpflrafreahgtayrfvgveidpkaldlp
pwaegiladfllwepgeafdlilgnppygivgeaskypihvfkavkdlykkafstwkgky
nlygaflekavrllkpggvlvfvvpatwlvledfallreflaregktsvyylgevfpqkk
vsavvirfqksgkglslwdtqesesgftpilwaeyphwegeiirfeteetrkleisgmpl
gdlfhirfaarspefkkhpavrkepgpglvpvltgrnlkpgwvdyeknhsglwmpkerak
elrdfyatphlvvahtkgtrvvaawderaypwreefhllpkegvrldpsslvqwlnseam
qkhvrtlyrdfvphltlrmlerlpvrreygfht

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.