PDB entry 2fmn

View 2fmn on RCSB PDB site
Description: Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase complex with LY309887
Class: oxidoreductase
Keywords: oxidoreductase, tim barrel, flavin, reductase
Deposited on 2006-01-09, released 2006-04-25
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.05 Å
R-factor: 0.217
AEROSPACI score: 0.42 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (176)
    Domains in SCOPe 2.07: d2fmna_
  • Chain 'B':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1
      • engineered (176)
    Domains in SCOPe 2.07: d2fmnb_
  • Chain 'C':
    Compound: 5,10-Methylenetetrahydrofolate reductase
    Species: Escherichia coli [TaxId:562]
    Gene: METF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEZ1 (Start-295)
      • engineered (176)
    Domains in SCOPe 2.07: d2fmnc_
  • Heterogens: FAD, 4HF, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2fmnA (A:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmnA (A:)
ffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtyga
nsgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgdlp
pemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnraitqfffd
vesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfdgldddae
trklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2fmnB (B:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmnB (B:)
ffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtyga
nsgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgdlp
pemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnraitqfffd
vesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfdgldddae
trklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2fmnC (C:)
msffhasqrdalnqslaevqgqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvty
gansgerdrthsiikgikdrtgleaaphltcidatpdelrtiardywnngirhivalrgd
lppgsgkpemyasdlvtllkevadfdisvaaypevhpeaksaqadllnlkrkvdagvnra
itqfffdvesylrfrdrcvsagidveiipgilpvsnfkqakkfadmtnvripawmaqmfd
gldddaetrklvganiamdmvkilsregvkdfhfytlnraemsyaichtlgvrpgllehh
hhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2fmnC (C:)
gqinvsfeffpprtsemeqtlwnsidrlsslkpkfvsvtygansgerdrthsiikgikdr
tgleaaphltcidatpdelrtiardywnngirhivalrgdlppemyasdlvtllkevadf
disvaaypevhpeaksaqadllnlkrkvdagvnraitqfffdvesylrfrdrcvsagidv
eiipgilpvsnfkqakkfadmtnvripawmaqmfdgldddaetrklvganiamdmvkils
regvkdfhfytlnraemsyaichtlgvrpgl