PDB entry 2c7f

View 2c7f on RCSB PDB site
Description: the structure of a family 51 arabinofuranosidase, araf51, from clostridium thermocellum in complex with 1,5-alpha-l-arabinotriose.
Class: hydrolase
Keywords: arabinofuranosidase, glycosidase, xylan, arabinan, hydrolase
Deposited on 2005-11-23, released 2005-12-14
The last revision prior to the SCOP 1.73 freeze date was dated 2006-12-20, with a file datestamp of 2007-06-28.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.24287
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7fa1, d2c7fa2
  • Chain 'B':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7fb1, d2c7fb2
  • Chain 'C':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7fc1, d2c7fc2
  • Chain 'D':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7fd1, d2c7fd2
  • Chain 'E':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7fe1, d2c7fe2
  • Chain 'F':
    Compound: alpha-l-arabinofuranosidase
    Species: CLOSTRIDIUM THERMOCELLUM
    Database cross-references and differences (RAF-indexed):
    • PDB 2C7F
      • engineered mutation (182)
    • Uniprot Q4CJG5
    Domains in SCOP 1.73: d2c7ff1, d2c7ff2
  • Heterogens: EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2c7fA (A:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fA (A:)
kkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkel
nvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaei
mmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvg
hktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyisl
hqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnn
edanimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapiv
ternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneeke
evtifavnrnihedivlvsdvrgmrllehivlehqdlkirnsvngeevypknsdkfddgi
ltsmlrraswnvirig

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2c7fB (B:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fB (B:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgmrllehivlehqdlkirnsvngeevypknsdkssfddg
iltsmlrraswnvirig

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2c7fC (C:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fC (C:)
armtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkelnv
piirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeimm
avnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvghk
tmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislhq
yygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnned
animqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivte
rnggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekeev
tifavnrnihedivlvsdvrgmrllehivlehqdlkirnsvngeevypknsdkssfddgi
ltsmlrraswnvirig

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2c7fD (D:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fD (D:)
armtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkelnv
piirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeimm
avnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvghk
tmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislhq
yygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnned
animqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivte
rnggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekeev
tifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvngeevypknsdkssfd
dgiltsmlrraswnvirig

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2c7fE (E:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fE (E:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgrllehivlehqdlkirnsvngeevypknsdkssiltsm
lrraswnvirig

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2c7fF (F:)
mgsshhhhhhmkkarmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgf
rkdvielvkelnvpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginef
akwckkvnaeimmavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwcl
gnamdgpwqvghktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvld
yaydyvdyislhqyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsf
dewnvwyhsnnedanimqnepwriappllediytfedallvglmlitlmkhadrikiacl
aqlinviapivternggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdi
esvaiyneekeevtifavnrnihedivlvsdvrgmkdyrllehivlehqdlkirnsvnge
evypknsdkssfddgiltsmlrraswnvirigk

    Sequence, based on observed residues (ATOM records): (download) 
    >2c7fF (F:)
karmtvdkdykiaeidkriygsfvehlgravydglyqpgnsksdedgfrkdvielvkeln
vpiirypggnfvsnyfwedgvgpvedrprrldlawksiepnqvginefakwckkvnaeim
mavnlgtrgisdacnlleycnhpggskysdmrikhgvkephnikvwclgnamdgpwqvgh
ktmdeygriaeetaramkmidpsielvacgssskdmptfpqweatvldyaydyvdyislh
qyygnkendtadflaksddlddfirsviatcdyikakkrskkdiylsfdewnvwyhsnne
danimqnepwriappllediytfedallvglmlitlmkhadrikiaclaqlinviapivt
ernggaawrqtifypfmhaskygrgivlqpvinsplhdtskhedvtdiesvaiyneekee
vtifavnrnihedivlvsdvrgmkrllehivlehqdlkirnsvngeevypknsdksfddg
iltsmlrraswnviri