PDB entry 2c4b

View 2c4b on RCSB PDB site
Description: Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A
Class: fusion protein
Keywords: squash inhibitor, hybrid microprotein, fusion protein, ribonuclease, endonuclease, hydrolase, nuclease, protease inhibitor, serine protease inhibitor
Deposited on 2005-10-18, released 2005-11-21
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-05-08, with a file datestamp of 2019-05-03.
Experiment type: XRAY
Resolution: 1.3 Å
R-factor: N/A
AEROSPACI score: 0.59 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: barnase mcoeeti fusion
    Species: ECBALLIUM ELATERIUM, synthetic [TaxId:3679]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-108)
      • linker (109-112)
      • engineered mutation (100)
    • Uniprot P82409 (113-128)
    • Uniprot P12071 (129-142)
    Domains in SCOPe 2.08: d2c4ba1, d2c4ba2
  • Chain 'B':
    Compound: barnase mcoeeti fusion
    Species: ECBALLIUM ELATERIUM, synthetic [TaxId:3679]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-108)
      • linker (109-112)
      • engineered mutation (100)
    • Uniprot P82409 (113-128)
    • Uniprot P12071 (129-142)
    Domains in SCOPe 2.08: d2c4bb1, d2c4bb2
  • Heterogens: 2PE, GOL, EDO, FMT, SO4, UNX, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2c4bA (A:)
qvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnreg
klpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkirsssmgvcpkil
kkcrrdsdclagcvcgpngfcgs

    Sequence, based on observed residues (ATOM records): (download) 
    >2c4bA (A:)
vintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnregk
lpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkirsssmgvcpkilk
kcrrdsdclagcvcgpngfcgs

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2c4bB (B:)
qvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnreg
klpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkirsssmgvcpkil
kkcrrdsdclagcvcgpngfcgs

    Sequence, based on observed residues (ATOM records): (download) 
    >2c4bB (B:)
vintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnregk
lpgksgrtwreadinytsgfrnsdrilyssdwliykttdayqtftkirsssmgvcpkilk
kcrrdsdclagcvcgpngfcgs