PDB entry 2bif

View 2bif on RCSB PDB site
Description: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase h256a mutant with f6p in phosphatase active site
Class: transferase, hydrolase
Keywords: kinase, transferase (phospho), phosphatase, hydrolase (phospho), glycolysis, bifunctional enzyme, transferase, hydrolase
Deposited on 1998-10-26, released 1999-02-16
The last revision prior to the SCOPe 2.06 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.2
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: protein (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase)
    Species: Rattus norvegicus [TaxId:10116]
    Gene: RT2K
    Database cross-references and differences (RAF-indexed):
    • Uniprot P25114 (Start-468)
      • engineered (64)
      • engineered (256)
      • engineered (299)
      • engineered (320)
    Domains in SCOPe 2.06: d2bifa1, d2bifa2
  • Chain 'B':
    Compound: protein (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase)
    Species: Rattus norvegicus [TaxId:10116]
    Gene: RT2K
    Database cross-references and differences (RAF-indexed):
    • Uniprot P25114 (Start-468)
      • engineered (64)
      • engineered (256)
      • engineered (299)
      • engineered (320)
    Domains in SCOPe 2.06: d2bifb1, d2bifb2
  • Heterogens: BOG, F6P, MG, PO4, ANP, SIN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2bifA (A:)
maspreltqnplkkifmpysngrpalhasqrgvcmtncptlivmvglpargktyiskklt
rylnfigvptrefnvgqyrrdmvktyksfefflpdneeglkirkqcalaalndvrkflse
egghvavfdatnttrerramifnfgeqngyktffvesicvdpeviaanivqvklgspdyv
nrdsdeatedfmrriecyensyesldeeqdrdlsyikimdvgqsyvvnrvadhiqsrivy
ylmnihvtprsiylcrageselnlkgriggdpglsprgrefskhlaqfisdqnikdlkvf
tsqmkrtiqtaealsvpyeqfkvlneidagvceemtyeeiqdhyplefalrdqdkyryry
pkgesyedlvqrlepvimelerqenvlvichqavmrcllayfldkaaeelpylkcplhtv
lkltpvaygckvesiflnvaavnthrdrpqnvdisrpseealvtvpahq

    Sequence, based on observed residues (ATOM records): (download) 
    >2bifA (A:)
cptlivmvglpargktyiskkltrylnfigvptrefnvgqyrrdmvktyksfefflpdne
eglkirkqcalaalndvrkflseegghvavfdatnttrerramifnfgeqngyktffves
icvdpeviaanivqvklgspdyvnrdsdeatedfmrriecyensyesldeeqdrdlsyik
imdvgqsyvvnrvadhiqsrivyylmnihvtprsiylcrageselnlkgriggdpglspr
grefskhlaqfisdqnikdlkvftsqmkrtiqtaealsvpyeqfkvlneidagvceemty
eeiqdhyplefalrdqdkyryrypkgesyedlvqrlepvimelerqenvlvichqavmrc
llayfldkaaeelpylkcplhtvlkltpvaygckvesiflnvaavnthrdrpqnvdisrp
seealvtvpahq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2bifB (B:)
maspreltqnplkkifmpysngrpalhasqrgvcmtncptlivmvglpargktyiskklt
rylnfigvptrefnvgqyrrdmvktyksfefflpdneeglkirkqcalaalndvrkflse
egghvavfdatnttrerramifnfgeqngyktffvesicvdpeviaanivqvklgspdyv
nrdsdeatedfmrriecyensyesldeeqdrdlsyikimdvgqsyvvnrvadhiqsrivy
ylmnihvtprsiylcrageselnlkgriggdpglsprgrefskhlaqfisdqnikdlkvf
tsqmkrtiqtaealsvpyeqfkvlneidagvceemtyeeiqdhyplefalrdqdkyryry
pkgesyedlvqrlepvimelerqenvlvichqavmrcllayfldkaaeelpylkcplhtv
lkltpvaygckvesiflnvaavnthrdrpqnvdisrpseealvtvpahq

    Sequence, based on observed residues (ATOM records): (download) 
    >2bifB (B:)
cptlivmvglpargktyiskkltrylnfigvptrefnvgqyrrdmvktyksfefflpdne
eglkirkqcalaalndvrkflseegghvavfdatnttrerramifnfgeqngyktffves
icvdpeviaanivqvklgspdyvnrdsdeatedfmrriecyensyesldeeqdrdlsyik
imdvgqsyvvnrvadhiqsrivyylmnihvtprsiylcrageselnlkgriggdpglspr
grefskhlaqfisdqnikdlkvftsqmkrtiqtaealsvpyeqfkvlneidagvceemty
eeiqdhyplefalrdqdkyryrypkgesyedlvqrlepvimelerqenvlvichqavmrc
llayfldkaaeelpylkcplhtvlkltpvaygckvesiflnvaavnthrdrpqnvdisrp
seealvtvpahq