PDB entry 1uko

View 1uko on RCSB PDB site
Description: Crystal structure of soybean beta-amylase mutant substituted at surface region
Class: hydrolase
Keywords: (alpha/beta)8 barrel, hydrolase
Deposited on 2003-08-30, released 2004-02-10
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.183
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-amylase
    Species: Glycine max [TaxId:3847]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10538 (Start-494)
      • see remark 999 (75)
      • see remark 999 (201)
      • engineered (373)
      • see remark 999 (398)
      • engineered (480)
      • engineered (486)
    Domains in SCOPe 2.07: d1ukoa_
  • Chain 'B':
    Compound: beta-amylase
    Species: Glycine max [TaxId:3847]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10538 (Start-494)
      • see remark 999 (75)
      • see remark 999 (201)
      • engineered (373)
      • see remark 999 (398)
      • engineered (480)
      • engineered (486)
    Domains in SCOPe 2.07: d1ukob_
  • Chain 'C':
    Compound: beta-amylase
    Species: Glycine max [TaxId:3847]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10538 (Start-494)
      • see remark 999 (75)
      • see remark 999 (201)
      • engineered (373)
      • see remark 999 (398)
      • engineered (480)
      • engineered (486)
    Domains in SCOPe 2.07: d1ukoc_
  • Chain 'D':
    Compound: beta-amylase
    Species: Glycine max [TaxId:3847]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10538 (Start-494)
      • see remark 999 (75)
      • see remark 999 (201)
      • engineered (373)
      • see remark 999 (398)
      • engineered (480)
      • engineered (486)
    Domains in SCOPe 2.07: d1ukod_
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1ukoA (A:)
atsdsnmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiie
lkgpkqydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhd
ifytnrsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievg
lgpagelrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkynd
vpestgffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgih
wwykvenhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpq
elvqqvlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylr
lsddllqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkpt
rpfpwldetdmkvdg

    Sequence, based on observed residues (ATOM records): (download) 
    >1ukoA (A:)
nmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiielkgpk
qydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhdifytn
rsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievglgpag
elrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkyndvpest
gffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgihwwykv
enhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpqelvqq
vlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylrlsddl
lqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkptrpfpw
ldetdmkvdg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1ukoB (B:)
atsdsnmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiie
lkgpkqydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhd
ifytnrsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievg
lgpagelrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkynd
vpestgffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgih
wwykvenhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpq
elvqqvlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylr
lsddllqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkpt
rpfpwldetdmkvdg

    Sequence, based on observed residues (ATOM records): (download) 
    >1ukoB (B:)
nmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiielkgpk
qydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhdifytn
rsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievglgpag
elrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkyndvpest
gffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgihwwykv
enhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpqelvqq
vlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylrlsddl
lqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkptrpfpw
ldetdmkvdg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1ukoC (C:)
atsdsnmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiie
lkgpkqydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhd
ifytnrsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievg
lgpagelrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkynd
vpestgffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgih
wwykvenhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpq
elvqqvlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylr
lsddllqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkpt
rpfpwldetdmkvdg

    Sequence, based on observed residues (ATOM records): (download) 
    >1ukoC (C:)
nmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiielkgpk
qydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhdifytn
rsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievglgpag
elrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkyndvpest
gffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgihwwykv
enhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpqelvqq
vlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylrlsddl
lqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkptrpfpw
ldetdmkvdg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1ukoD (D:)
atsdsnmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiie
lkgpkqydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhd
ifytnrsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievg
lgpagelrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkynd
vpestgffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgih
wwykvenhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpq
elvqqvlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylr
lsddllqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkpt
rpfpwldetdmkvdg

    Sequence, based on observed residues (ATOM records): (download) 
    >1ukoD (D:)
nmllnyvpvyvmlplgvvnvdnvfedpdglkeqllqlraagvdgvmvdvwwgiielkgpk
qydwrayrsllqlvqecgltlqaimsfhqcggnvgdivnipipqwvldigesnhdifytn
rsgtrnkeyltvgvdnepifhgrtaieiysdymksfrenmsdflesgliidievglgpag
elrypsypqsqgwefpgigefqcydkylkadfkaavaraghpewelpddagkyndvpest
gffksngtyvtekgkffltwysnkllnhgdqildeankaflgckvklaikvsgihwwykv
enhaaeltagyynlndrdgyrpiarmlsrhhailnftclemrdseqpsdaksgpqelvqq
vlsggwreyirvagenalprydataynqiilnarpqgvnnngppklsmfgvtylrlsddl
lqksnfnifkkfvlkmhadqdycanpqkynhaitplkpsapkipievlleatkptrpfpw
ldetdmkvdg