PDB entry 1hl2

View 1hl2 on RCSB PDB site
Description: crystal structure of n-acetylneuraminate lyase from e. coli mutant l142r in complex with b-hydroxypyruvate
Class: lyase
Keywords: n-acetylneuraminate lyase, class I aldolase, lyase, carbohydrate metabolism, schiff base
Deposited on 2003-03-12, released 2003-05-09
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.183
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase subunit
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • PDB 1HL2
      • engineered mutation (141)
    • Uniprot P06995 (Start-296)
    Domains in SCOPe 2.07: d1hl2a_
  • Chain 'B':
    Compound: n-acetylneuraminate lyase subunit
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • PDB 1HL2
      • engineered mutation (141)
    • Uniprot P06995
    Domains in SCOPe 2.07: d1hl2b_
  • Chain 'C':
    Compound: n-acetylneuraminate lyase subunit
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • PDB 1HL2
      • engineered mutation (141)
    • Uniprot P06995 (Start-296)
    Domains in SCOPe 2.07: d1hl2c_
  • Chain 'D':
    Compound: n-acetylneuraminate lyase subunit
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • PDB 1HL2
      • engineered mutation (141)
    • Uniprot P06995 (Start-296)
    Domains in SCOPe 2.07: d1hl2d_
  • Heterogens: 3PY, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1hl2A (A:)
matnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsere
qvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcd
hyraiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirre
hpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecn
kvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >1hl2A (A:)
tnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqv
leivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhy
raiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehp
dlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkv
idlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1hl2B (B:)
matnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsere
qvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcd
hyraiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirre
hpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecn
kvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >1hl2B (B:)
tnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqv
leivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhy
raiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehp
dlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkv
idlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1hl2C (C:)
matnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsere
qvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcd
hyraiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirre
hpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecn
kvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >1hl2C (C:)
tnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqv
leivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhy
raiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehp
dlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkv
idlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >1hl2D (D:)
matnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsere
qvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcd
hyraiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirre
hpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecn
kvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >1hl2D (D:)
tnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqv
leivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhy
raiidsadglpmvvyniparsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehp
dlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkv
idlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg