SCOPe 2.07: Structural Classification of Proteins

PDB entry 7m32

View 7m32 on RCSB PDB site
Description: Dihydropyrimidine Dehydrogenase (DPD) C671A Mutant Soaked with Uracil and NADPH Anaerobically
Class: FLAVOPROTEIN, Oxidoreductase
Keywords: Dihydropyrimidine Dehydrogenase, DPD, mutant, uracil, flavin, soaking, ligand, FMN, FAD, FLAVOPROTEIN, Oxidoreductase
Deposited on 2021-03-18, released 2021-06-09
The last revision prior to the SCOPe 2.07 freeze date was dated 2021-06-23, with a file datestamp of 2021-06-18.
Experiment type: XRAY
Resolution: 1.82 Å
R-factor: N/A
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Dihydropyrimidine dehydrogenase [NADP(+)]
Species: Sus scrofa [TaxId:9823]
Gene: DPYD
Database cross-references and differences (RAF-indexed):
- Uniprot Q28943
  - conflict (59)
  - engineered mutation (670)
Domains in SCOPe 2.07: d7m32a1, d7m32a2, d7m32a3, d7m32a4, d7m32a5
Chain 'B':
Compound: Dihydropyrimidine dehydrogenase [NADP(+)]
Species: Sus scrofa [TaxId:9823]
Gene: DPYD
Database cross-references and differences (RAF-indexed):
- Uniprot Q28943
  - conflict (59)
  - engineered mutation (670)
Domains in SCOPe 2.07: d7m32b1, d7m32b2, d7m32b3, d7m32b4, d7m32b5
Chain 'C':
Compound: Dihydropyrimidine dehydrogenase [NADP(+)]
Species: Sus scrofa [TaxId:9823]
Gene: DPYD
Database cross-references and differences (RAF-indexed):
- Uniprot Q28943
  - conflict (59)
  - engineered mutation (670)
Domains in SCOPe 2.07: d7m32c1, d7m32c2, d7m32c3, d7m32c4, d7m32c5
Chain 'D':
Compound: Dihydropyrimidine dehydrogenase [NADP(+)]
Species: Sus scrofa [TaxId:9823]
Gene: DPYD
Database cross-references and differences (RAF-indexed):
- Uniprot Q28943
  - conflict (59)
  - engineered mutation (670)
Domains in SCOPe 2.07: d7m32d1, d7m32d2, d7m32d3, d7m32d4, d7m32d5
Heterogens: ALA, FAD, FNR, LEU, PRO, SF4, URA, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>7m32A (A:)
mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
vnpvc

Sequence, based on observed residues (ATOM records): (download)

>7m32A (A:)
pvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfddi
khttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnpl
gltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqekm
peaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvvn
feielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdqg
fytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvflv
frkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwned
edqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggdi
vgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglkf
inpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmygp
gqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasga
dalelnlsaphgglacgqdpelvrnicrwvrqavqipffakltpnvtdivsiaraakegg
adgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravttiaralpgf
pilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylksieelqg
wdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqnaafpple
rkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcymtcndsgy
qaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpl

Chain 'B':
Sequence, based on SEQRES records: (download)

>7m32B (B:)
mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
vnpvc

Sequence, based on observed residues (ATOM records): (download)

>7m32B (B:)
pvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfddi
khttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnpl
gltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqekm
peaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvvn
feielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdqg
fytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvflv
frkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwned
edqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggdi
vgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglkf
inpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmygp
gqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasga
dalelnlsaphmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsiaraakegg
adgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravttiaralpgf
pilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylksieelqg
wdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqnaafpple
rkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcymtcndsgy
qaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglp

Chain 'C':
Sequence, based on SEQRES records: (download)

>7m32C (C:)
mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
vnpvc

Sequence, based on observed residues (ATOM records): (download)

>7m32C (C:)
pvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfddi
khttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnpl
gltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqekm
peaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvvn
feielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdqg
fytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvflv
frkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwned
edqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggdi
vgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglkf
inpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmygp
gqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasga
dalelnlsaphlacgqdpelvrnicrwvrqavqipffakltpnvtdivsiaraakeggad
gvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravttiaralpgfpi
latggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylksieelqgwd
gqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqnaafpplerk
pfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcymtcndsgyqa
iqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrgl

Chain 'D':
Sequence, based on SEQRES records: (download)

>7m32D (D:)
mapvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfd
dikhttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdn
plgltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqe
kmpeaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydv
vnfeielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqd
qgfytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvf
lvfrkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwn
ededqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfagg
divgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemagl
kfinpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmy
gpgqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeas
gadalelnlsaphgmgergmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsi
araakeggadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravtt
iaralpgfpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallyl
ksieelqgwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeq
naafpplerkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcy
mtcndsgyqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpla
vnpvc

Sequence, based on observed residues (ATOM records): (download)

>7m32D (D:)
pvlskdvadiesilalnprtqshaalhstlakkldkkhwkrnpdkncfhceklennfddi
khttlgergalreamrclkcadapcqkscpthldiksfitsisnknyygaakmifsdnpl
gltcgmvcptsdlcvggcnlyateegsinigglqqfasevfkamnipqirnpclpsqekm
peaysakiallgagpasiscasflarlgysditifekqeyvgglstseipqfrlpydvvn
feielmkdlgvkiicgkslseneitlntlkeegykaafigiglpepktddifqgltqdqg
fytskdflplvaksskagmcachsplpsirgavivlgagdtafdcatsalrcgarrvflv
frkgfvniravpeevelakeekceflpflsprkvivkggrivavqfvrteqdetgkwned
edqivhlkadvvisafgsvlrdpkvkealspikfnrwdlpevdpetmqtsepwvfaggdi
vgmanttvesvndgkqaswyihkyiqaqygasvsakpelplfytpvdlvdisvemaglkf
inpfglasaapttsssmirrafeagwgfaltktfsldkdivtnvsprivrgttsgpmygp
gqssflnielisektaaywcqsvtelkadfpdniviasimcsynkndwmelsrkaeasga
dalelnlsaphgmglacgqdpelvrnicrwvrqavqipffakltpnvtdivsiaraakeg
gadgvtatntvsglmglkadgtpwpavgagkrttyggvsgtairpialravttiaralpg
fpilatggidsaesglqflhsgasvlqvcsavqnqdftviqdyctglkallylksieelq
gwdgqspgteshqkgkpvpriaelmgkklpnfgpyleqrkkiiaeekmrlkeqnaafppl
erkpfipkkpipaikdvigkalqylgtfgelsnieqvvavideemcincgkcymtcndsg
yqaiqfdpethlptvtdtctgctlclsvcpiidcirmvsrttpyepkrglpl