PDB entry 7ea9

View 7ea9 on RCSB PDB site
Description: Crystal Structure of human lysyl-tRNA synthetase Y145H mutant
Class: ligase
Keywords: lysyl-tRNA synthetase, LysRS, disease related mutant, LIGASE
Deposited on 2021-03-06, released 2021-03-31
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-05-05, with a file datestamp of 2021-04-30.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lysine--tRNA ligase
    Species: Homo sapiens [TaxId:9606]
    Gene: KARS1, KARS, KIAA0070
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q15046
      • engineered mutation (76)
    Domains in SCOPe 2.08: d7ea9a1, d7ea9a2
  • Chain 'B':
    Compound: Lysine--tRNA ligase
    Species: Homo sapiens [TaxId:9606]
    Gene: KARS1, KARS, KIAA0070
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q15046
      • engineered mutation (76)
    Domains in SCOPe 2.08: d7ea9b1, d7ea9b2
  • Chain 'C':
    Compound: Lysine--tRNA ligase
    Species: Homo sapiens [TaxId:9606]
    Gene: KARS1, KARS, KIAA0070
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q15046
      • engineered mutation (76)
    Domains in SCOPe 2.08: d7ea9c1, d7ea9c2
  • Chain 'D':
    Compound: Lysine--tRNA ligase
    Species: Homo sapiens [TaxId:9606]
    Gene: KARS1, KARS, KIAA0070
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q15046
      • engineered mutation (76)
    Domains in SCOPe 2.08: d7ea9d1, d7ea9d2
  • Heterogens: KAA, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7ea9A (A:)
msvdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkv
agrihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnp
gktkkgelsiipyeitllspclhmlphlhfglkdketryrqryldlilndfvrqkfiirs
kiityirsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlv
vggidrvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgs
ykvtyhpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicv
akavecppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfel
fvmkkeicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwg
mgidrvamfltdsnnikevllfpamkpedkkenlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7ea9A (A:)
vdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkvag
rihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnpgk
tkkgelsiipyeitllspclhmlphglkdketryrqryldlilndfvrqkfiirskiity
irsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlvvggid
rvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgsykvty
hpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicvakave
cppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfelfvmkk
eicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwgmgidr
vamfltdsnnikevllfpamkp

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7ea9B (B:)
msvdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkv
agrihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnp
gktkkgelsiipyeitllspclhmlphlhfglkdketryrqryldlilndfvrqkfiirs
kiityirsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlv
vggidrvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgs
ykvtyhpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicv
akavecppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfel
fvmkkeicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwg
mgidrvamfltdsnnikevllfpamkpedkkenlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7ea9B (B:)
dpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkvagr
ihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnpgkt
kkgelsiipyeitllspclhmlphlkdketryrqryldlilndfvrqkfiirskiityir
sfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlvvggidrv
yeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgsykvtyhp
dgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicvakavecp
pprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfelfvmkkei
cnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwgmgidrva
mfltdsnnikevllfpamkp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7ea9C (C:)
msvdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkv
agrihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnp
gktkkgelsiipyeitllspclhmlphlhfglkdketryrqryldlilndfvrqkfiirs
kiityirsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlv
vggidrvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgs
ykvtyhpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicv
akavecppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfel
fvmkkeicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwg
mgidrvamfltdsnnikevllfpamkpedkkenlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7ea9C (C:)
vdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkvag
rihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnpgk
tkkgelsiipyeitllspclhmlphglkdketryrqryldlilndfvrqkfiirskiity
irsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlvvggid
rvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgsykvty
hpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicvakave
cppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfelfvmkk
eicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwgmgidr
vamfltdsnnikevllfpamkp

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7ea9D (D:)
msvdpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkv
agrihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnp
gktkkgelsiipyeitllspclhmlphlhfglkdketryrqryldlilndfvrqkfiirs
kiityirsfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlv
vggidrvyeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgs
ykvtyhpdgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicv
akavecppprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfel
fvmkkeicnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwg
mgidrvamfltdsnnikevllfpamkpedkkenlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7ea9D (D:)
dpnqyykirsqaihqlkvngedpyphkfhvdisltdfiqkyshlqpgdhltditlkvagr
ihakrasggklifhdlrgegvklqvmansrnykseeefihinnklrrgdiigvqgnpgkt
kkgelsiipyeitllspclhmlpglkdketryrqryldlilndfvrqkfiirskiityir
sfldelgfleietpmmniipggavakpfityhneldmnlymriapelyhkmlvvggidrv
yeigrqfrnegidlthnpefttcefymayadyhdlmeitekmvsgmvkhitgsykvtyhp
dgpegqaydvdftppfrrinmveelekalgmklpetnlfeteetrkilddicvakavecp
pprttarlldklvgeflevtcinptficdhpqimsplakwhrskeglterfelfvmkkei
cnaytelndpmrqrqlfeeqakakaagddeamfidenfctaleyglpptagwgmgidrva
mfltdsnnikevllfpamkp