PDB entry 6mn0

View 6mn0 on RCSB PDB site
Description: Crystal structure of meta-AAC0038, an environmental aminoglycoside resistance enzyme, H168A mutant in complex with acetyl-CoA
Class: transferase
Keywords: ANTIBIOTIC_NAT FAMILY, ACETYLTRANSFERASE, AMINOGLYCOSIDE, ANTIBIOTIC RESISTANCE, METAGENOME, SOIL, COENZYME A, gentamicin, CSGID, TRANSFERASE, CENTER FOR STRUCTURAL GENOMICS OF INFECTIOUS DISEASES, National Institute of Allergy and Infectious Diseases, NIAID
Deposited on 2018-10-01, released 2018-10-24
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: N/A
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059X981 (Start-262)
      • engineered mutation (167)
    Domains in SCOPe 2.08: d6mn0a_
  • Chain 'B':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059X981 (Start-262)
      • engineered mutation (167)
    Domains in SCOPe 2.08: d6mn0b_
  • Chain 'C':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059X981 (1-End)
      • engineered mutation (167)
    Domains in SCOPe 2.08: d6mn0c_
  • Chain 'D':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6mn0d_
  • Chain 'E':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059X981 (Start-262)
      • engineered mutation (167)
    Domains in SCOPe 2.08: d6mn0e_
  • Chain 'F':
    Compound: Aminoglycoside N(3)-acetyltransferase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059X981 (Start-262)
      • engineered mutation (167)
    Domains in SCOPe 2.08: d6mn0f_
  • Heterogens: ACO, CL, GOL, SO4, PE3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6mn0A (A:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0A (A:)
rvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlgya
dwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaaigg
eaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkrilr
yeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvlvp
adeivafavdwlerwgrtar

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6mn0B (B:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0B (B:)
rvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlgya
dwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaaigg
eaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkrilr
yeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvlvp
adeivafavdwlerwgrtar

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6mn0C (C:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0C (C:)
ssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlg
yadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaai
ggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkri
lryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvl
vpadeivafavdwlerwgrta

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6mn0D (D:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0D (D:)
srvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlgy
adwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaaig
geaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkril
ryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvlv
padeivafavdwlerwgrta

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >6mn0E (E:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0E (E:)
rvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlgya
dwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaaigg
eaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkrilr
yeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvlvp
adeivafavdwlerwgrtar

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >6mn0F (F:)
vssrvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvl
gyadwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaa
iggeaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkr
ilryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassv
lvpadeivafavdwlerwgrtar

    Sequence, based on observed residues (ATOM records): (download) 
    >6mn0F (F:)
srvstrsslaedlraigladgdavlvhaalrkvgkivggpddildamrdvigpagtvlgy
adwqledeirddpamrehipafdplrsrsirdngfwpelirttpgalrsaspgasmaaig
geaewftadhaldygygprsplgklveakgkvlmlgapldtmtllahaehladfpnkril
ryeapilvdgekvwrwfeefdtsdppdgladdyfagiveeflatgrgkrgkigeassvlv
padeivafavdwlerwgrtar