PDB entry 6m4m

View 6m4m on RCSB PDB site
Description: X-ray crystal structure of the E249Q mutan of alpha-amylase I and maltohexaose complex from Eisenia fetida
Class: hydrolase
Keywords: TIM barrel, GH family 13, carbohydrase, cold-active, HYDROLASE
Deposited on 2020-03-07, released 2020-09-16
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-09-16, with a file datestamp of 2020-09-11.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: N/A
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alpha-amylase
    Species: Eisenia fetida [TaxId:6396]
    Gene: alpha-amylase
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A173N065 (4-496)
      • expression tag (3)
      • engineered mutation (235)
      • expression tag (497-498)
    Domains in SCOPe 2.08: d6m4ma1, d6m4ma2, d6m4ma3, d6m4ma4
  • Heterogens: CA, GLC, CL, SO4, PG4, PGE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6m4mA (A:)
eaefqyfgsyycqpgrdvivhlfewkwtdierecqwladhnycgvqvsppnehrivtdpp
ypwwqryqpvsytmnsrsgsetqfrnmvttcnnlgvyiyvdgvlnhmtgggsgtgsdgns
fdgdslqypgvpygpndfhgsddcstsdgeihdynnptevrncrlsglrdldgsanyvrd
veaefanrligwgvagfrldackhmwpgdleailgrlnqlntqwfpagrnayiyqqvidl
ggeavkateytylgrvtefkhgqnlgnvvrknngqrlanlvnfgegwgqlsgfdalvfid
nhdnqrghgaggfgtiltffeanmykiatafelawdyghvrlmssynwprnivngvdtnd
wvgpptngggdtkdvecfngewicehrwremynmvrfhnvaignpvsnwwdngfqaiafg
rgnrgfifinnedfaitqtlqtglpggeycdviscdnnrppcgnsggacratvivngdgt
atfdvpngenpmiaihvfleqkliseedlnsavdhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4mA (A:)
fqyfgsyycqpgrdvivhlfewkwtdierecqwladhnycgvqvsppnehrivtdppypw
wqryqpvsytmnsrsgsetqfrnmvttcnnlgvyiyvdgvlnhmtgggsgtgsdgnsfdg
dslqypgvpygpndfhgsddcstsdgeihdynnptevrncrlsglrdldgsanyvrdvea
efanrligwgvagfrldackhmwpgdleailgrlnqlntqwfpagrnayiyqqvidlgge
avkateytylgrvtefkhgqnlgnvvrknngqrlanlvnfgegwgqlsgfdalvfidnhd
nqrghgaggfgtiltffeanmykiatafelawdyghvrlmssynwprnivngvdtndwvg
pptngggdtkdvecfngewicehrwremynmvrfhnvaignpvsnwwdngfqaiafgrgn
rgfifinnedfaitqtlqtglpggeycdviscdnnrppcgnsggacratvivngdgtatf
dvpngenpmiaihvfl