PDB entry 6j9s

View 6j9s on RCSB PDB site
Description: Penta mutant of Lactobacillus casei lactate dehydrogenase
Class: oxidoreductase
Keywords: oxidoreductase
Deposited on 2019-01-24, released 2019-02-06
The last revision prior to the SCOPe 2.07 freeze date was dated 2019-02-06, with a file datestamp of 2019-02-01.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9sa1, d6j9sa2
  • Chain 'B':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9sb1, d6j9sb2
  • Chain 'C':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9sc1, d6j9sc2
  • Chain 'D':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9sd1, d6j9sd2
  • Chain 'E':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9se1, d6j9se2
  • Chain 'F':
    Compound: l-lactate dehydrogenase
    Species: Lactobacillus casei subsp. casei ATCC 393 [TaxId:219334]
    Gene: ldh, LBCZ_2323
    Database cross-references and differences (RAF-indexed):
    • Uniprot S6C1Z0
      • engineered mutation (54-55)
      • engineered mutation (163)
      • engineered mutation (222-223)
    Domains in SCOPe 2.07: d6j9sf1, d6j9sf2
  • Heterogens: SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6j9sA (A:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sA (A:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitanlkilksivdpivdsgfngiflvaanpvdiltyatwkl
    sgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehgdtefpvwshaniggvt
    iaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiatalariskailndenavl
    plsvymdgqyglndiyigtpavinrngiqnileipltdheeesmqksasqlkkv
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >6j9sB (B:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sB (B:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngifl
    vaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehg
    dtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiata
    lariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheeesm
    qksasqlkkvltdaf
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >6j9sC (C:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sC (C:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitavnknlkilksivdpivdsgfngiflvaanpvdiltyat
    wklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehgdtefpvwshanig
    gvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiatalariskailnden
    avlplsvymdgqyglndiyigtpavinrngiqnileipltdheeesmqksasqlkkvlt
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >6j9sD (D:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sD (D:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitaknlkilksivdpivdsgfngiflvaanpvdiltyatwk
    lsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehgdtefpvwshaniggv
    tiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiatalariskailndenav
    lplsvymdgqyglndiyigtpavinrngiqnileipltdheeesmqksasqlkkvlt
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >6j9sE (E:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sE (E:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngifl
    vaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehg
    dtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiata
    lariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheeesm
    qksasqlkkvltdaf
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >6j9sF (F:)
    masitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpf
    tspkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngi
    flvaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimge
    hgdtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygia
    talariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheee
    smqksasqlkkvltdafakndietrq
    

    Sequence, based on observed residues (ATOM records): (download)
    >6j9sF (F:)
    sitdkdhqkvilvgdgavgssyayamvlqgiaqeigivdifkdktkgdaidledalpfts
    pkkiysaeysdakdadlvvitagapqkpgetrldlvnknlkilksivdpivdsgfngifl
    vaanpvdiltyatwklsgfpknrvvgsgtsldtarfrqsiakmvnvdarsvhayimgehg
    dtefpvwshaniggvtiaewvkahpeikedklvkmfedvrnkayeiiklkgatfygiata
    lariskailndenavlplsvymdgqyglndiyigtpavinrngiqnileipltdheeesm
    qksasqlkkvltdaf