PDB entry 6cuz

View 6cuz on RCSB PDB site
Description: Engineered TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3R)-ethylserine bound as the amino-acrylate
Class: lyase
Keywords: amino-acrylate, (2S, 3R)-ethylserine, LYASE
Deposited on 2018-03-27, released 2018-09-26
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-09-26, with a file datestamp of 2018-09-21.
Experiment type: XRAY
Resolution: 1.75 Å
R-factor: N/A
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (90)
      • engineered mutation (94)
      • engineered mutation (160)
      • engineered mutation (172)
      • engineered mutation (273)
      • engineered mutation (291)
    Domains in SCOPe 2.07: d6cuza_
  • Chain 'B':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (90)
      • engineered mutation (94)
      • engineered mutation (160)
      • engineered mutation (172)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (383)
    Domains in SCOPe 2.07: d6cuzb_
  • Chain 'C':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (90)
      • engineered mutation (94)
      • engineered mutation (172)
      • engineered mutation (273)
      • engineered mutation (291)
    Domains in SCOPe 2.07: d6cuzc_
  • Chain 'D':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (90)
      • engineered mutation (94)
      • engineered mutation (160)
      • engineered mutation (172)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (383)
    Domains in SCOPe 2.07: d6cuzd_
  • Heterogens: NA, FEV, PO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6cuzA (A:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >6cuzA (A:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlk
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >6cuzB (B:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >6cuzB (B:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymerqkmnvfrmkllganvipvnsrtakdainealrdweatfeythyligsvv
    gphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfvndkkvkl
    vgveaggkglesgkhsaslnagqvgvlhgmlsyflqdegqikpshsiapgldypgvgpeh
    aylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakemsrdeiiiv
    nlsgrgdkdldivlka
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >6cuzC (C:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >6cuzC (C:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsdainealrdweatfeythyligsv
    vgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfvndkkvk
    lvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgldypgvgp
    ehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakemsrdeii
    ivnlsgrgdkdldivlk
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >6cuzD (D:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnv
    

    Sequence, based on observed residues (ATOM records): (download)
    >6cuzD (D:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakiylkredlvhggahktnnaigqaplaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtakdainealrdweatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvlhgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvtvtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkas