PDB entry 6asm

View 6asm on RCSB PDB site
Description: E. coli phosphoenolpyruvate carboxykinase G209S K212C mutant bound to thiosulfate
Class: lyase
Keywords: Nonnative ligand, LYASE
Deposited on 2017-08-25, released 2018-08-29
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-08-29, with a file datestamp of 2018-08-24.
Experiment type: XRAY
Resolution: 1.55 Å
R-factor: N/A
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Phosphoenolpyruvate carboxykinase (ATP)
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: pckA, pck, b3403, JW3366
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22259 (Start-539)
      • engineered mutation (208)
      • engineered mutation (211)
      • expression tag (540-542)
    Domains in SCOPe 2.07: d6asma1, d6asma2, d6asma3
  • Heterogens: THJ, ATP, MG, MN, XE, BTB, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6asmA (A:)
    mrvnngltpqeleaygisdvhdivynpsydllyqeeldpsltgyergvltnlgavavdtg
    iftgrspkdkyivrddttrdtfwwadkgkgkndnkplspetwqhlkglvtrqlsgkrlfv
    vdafcganpdtrlsvrfitevawqahfvknmfirpsdeelagfkpdfivmngakctnpqw
    keqglnsenfvafnltermqliggtwygsemckgmfsmmnyllplkgiasmhcsanvgek
    gdvavffglsgtgkttlstdpkrrligddehgwdddgvfnfeggcyaktiklskeaepei
    ynairrdallenvtvredgtidfddgsktentrvsypiyhidnivkpvskaghatkvifl
    tadafgvlppvsrltadqtqyhflsgftaklagtergiteptptfsacfgaaflslhptq
    yaevlvkrmqaagaqaylvntgwngtgkrisikdtraiidailngsldnaetftlpmfnl
    aiptelpgvdtkildprntyaspeqwqekaetlaklfidnfdkytdtpagaalvaagpkl
    hhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6asmA (A:)
    ltpqeleaygisdvhdivynpsydllyqeeldpsltgyergvltnlgavavdtgiftgrs
    pkdkyivrddttrdtfwwadkgkndnkplspetwqhlkglvtrqlsgkrlfvvdafcgan
    pdtrlsvrfitevawqahfvknmfirpsdeelagfkpdfivmngakctnpqwkeqglnse
    nfvafnltermqliggtwygsemckgmfsmmnyllplkgiasmhcsanvgekgdvavffg
    lsgtgkttlstdpkrrligddehgwdddgvfnfeggcyaktiklskeaepeiynairrda
    llenvtvredgtidfddgsktentrvsypiyhidnivkpvskaghatkvifltadafgvl
    ppvsrltadqtqyhflsgftakleptptfsacfgaaflslhptqyaevlvkrmqaagaqa
    ylvntgwngtgkrisikdtraiidailngsldnaetftlpmfnlaiptelpgvdtkildp
    rntyaspeqwqekaetlaklfidnfdkytdtpagaalvaagpklhhh