PDB entry 6ami

View 6ami on RCSB PDB site
Description: Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB4D11 with Trp non-covalently bound
Class: biosynthetic protein
Keywords: PLP Type II, Tryptophan Synthase, Engineered, Allostery, BIOSYNTHETIC PROTEIN
Deposited on 2017-08-09, released 2018-05-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-05-16, with a file datestamp of 2018-05-11.
Experiment type: XRAY
Resolution: 1.97 Å
R-factor: N/A
AEROSPACI score: 0.32 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (16)
      • engineered mutation (67)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
    Domains in SCOPe 2.07: d6amia_
  • Chain 'B':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-387)
      • engineered mutation (16)
      • engineered mutation (67)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
    Domains in SCOPe 2.07: d6amib_
  • Chain 'C':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (16)
      • engineered mutation (67)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
    Domains in SCOPe 2.07: d6amic_
  • Chain 'D':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (16)
      • engineered mutation (67)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
    Domains in SCOPe 2.07: d6amid_
  • Heterogens: LLP, TRP, NA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6amiA (A:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkvsgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6amiA (A:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdgqikpshsiapgldyp
    gvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakemsr
    deiiivnlsgrgdkdldivlkvs
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >6amiB (B:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkvsgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6amiB (B:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdegqikpshsiapgldy
    pgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakems
    rdeiiivnlsgrgdkdldivlkvsgnv
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >6amiC (C:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkvsgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6amiC (C:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdegqikpshsiapgldy
    pgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakems
    rdeiiivnlsgrgdkdldivlkvsg
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >6amiD (D:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkvsgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6amiD (D:)
    mwfgefggqyvpetligplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallakfmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdegqikpshsiapgldy
    pgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakems
    rdeiiivnlsgrgdkdldivlkvs