PDB entry 6am9

View 6am9 on RCSB PDB site
Description: Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9, with Ser-bound in a predominantly closed state.
Class: lyase
Keywords: PLP Type II, Tryptophan Synthase, Engineered, Allostery, BIOSYNTHETIC PROTEIN, LYASE
Deposited on 2017-08-09, released 2018-05-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-05-16, with a file datestamp of 2018-05-11.
Experiment type: XRAY
Resolution: 2.09 Å
R-factor: N/A
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (67)
      • engineered mutation (94)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
      • engineered mutation (383)
    Domains in SCOPe 2.07: d6am9a_
  • Chain 'B':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (67)
      • engineered mutation (94)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
      • engineered mutation (383)
    Domains in SCOPe 2.07: d6am9b_
  • Chain 'C':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (67)
      • engineered mutation (94)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
    Domains in SCOPe 2.07: d6am9c_
  • Chain 'D':
    Compound: Tryptophan synthase beta chain 1
    Species: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [TaxId:186497]
    Gene: trpB1, PF1706
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8U093 (0-End)
      • engineered mutation (15-16)
      • engineered mutation (67)
      • engineered mutation (94)
      • engineered mutation (273)
      • engineered mutation (291)
      • engineered mutation (320)
      • engineered mutation (383)
    Domains in SCOPe 2.07: d6am9d_
  • Heterogens: 0JO, PLS, NA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6am9A (A:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6am9A (A:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkas
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >6am9B (B:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6am9B (B:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdgqikpshsiapgldyp
    gvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakemsr
    deiiivnlsgrgdkdldivlkas
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >6am9C (C:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6am9C (C:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlk
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >6am9D (D:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkasgnvlehhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6am9D (D:)
    mwfgefggqyvpetlvgplkelekaykrfkddeefnrqlnyylktwagrptplyyakrlt
    ekiggakvylkredlvhggahktnnaigqallaklmgktrliaetgagqhgvatamagal
    lgmkvdiymgaedverqkmnvfrmkllganvipvnsgsrtlkdainealrdwvatfeyth
    yligsvvgphpyptivrdfqsvigreakaqileaegqlpdvivacvgggsnamgifypfv
    ndkkvklvgveaggkglesgkhsaslnagqvgvshgmlsyflqdeegqikpshsiapgld
    ypgvgpehaylkkiqraeyvavtdeealkafhelsrtegiipalesahavayamklakem
    srdeiiivnlsgrgdkdldivlkas