PDB entry 5tdh

View 5tdh on RCSB PDB site
Description: The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S
Class: cell cycle
Keywords: dominant negative, G-alpha(i)-1-beta-1-gamma-2 heterotrimer, G203A, A326S, GPCR, GDP, CELL CYCLE
Deposited on 2016-09-19, released 2016-11-09
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-09-27, with a file datestamp of 2017-09-22.
Experiment type: XRAY
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(i) subunit alpha-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAI1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63096
      • conflict (97)
      • engineered mutation (202)
      • engineered mutation (325)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Rattus norvegicus [TaxId:10116]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P54311 (2-341)
      • expression tag (0-1)
    Domains in SCOPe 2.08: d5tdhb1, d5tdhb2
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5tdhg_
  • Chain 'H':
    Compound: Guanine nucleotide-binding protein G(i) subunit alpha-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAI1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63096
      • conflict (97)
      • engineered mutation (202)
      • engineered mutation (325)
  • Chain 'J':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Rattus norvegicus [TaxId:10116]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P54311 (2-341)
      • expression tag (0-1)
    Domains in SCOPe 2.08: d5tdhj1, d5tdhj2
  • Chain 'K':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5tdhk_
  • Heterogens: GDP

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5tdhB (B:)
samseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlaki
yamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggld
nicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtt
tftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpng
nafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwd
alkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records): (download) 
    >5tdhB (B:)
samseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlaki
yamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggld
nicsiynlkgnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >5tdhG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffc

    Sequence, based on observed residues (ATOM records): (download) 
    >5tdhG (G:)
siaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'H':
    No sequence available.

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >5tdhJ (J:)
samseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlaki
yamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggld
nicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtt
tftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpng
nafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwd
alkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records): (download) 
    >5tdhJ (J:)
samseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlaki
yamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggld
nicsiynlkgnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >5tdhK (K:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffc

    Sequence, based on observed residues (ATOM records): (download) 
    >5tdhK (K:)
siaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr