PDB entry 5mgd

View 5mgd on RCSB PDB site
Description: STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose
Class: hydrolase
Keywords: TIM barrel, GH35, GLYCOSIDE HYDROLASE, KINETICS, PROTEIN CONFORMATION, carbohydrate metabolism, b-galactosidase, Aspergillus niger, fungal protein, SUBSTRATE SPECIFICITY, prebiotics, galactooligosaccharides, GOS, recombinant, 6-Gal-Lac, 6-Galactosyllactose, HYDROLASE
Deposited on 2016-11-21, released 2017-04-19
The last revision prior to the SCOPe 2.06 freeze date was dated 2017-04-19, with a file datestamp of 2017-04-14.
Experiment type: XRAY
Resolution: 2.15 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Probable beta-galactosidase A
    Species: Aspergillus niger CBS 513.88 [TaxId:425011]
    Gene: lacA, An01g12150
    Database cross-references and differences (RAF-indexed):
    • Uniprot A2QAN3 (Start-1006)
      • engineered mutation (297)
      • expression tag (1007)
    Domains in SCOPe 2.06: d5mgda1, d5mgda2, d5mgda3, d5mgda4, d5mgda5, d5mgda6
  • Heterogens: NAG, BMA, MAN, GLC, GAL, DMS, CL, 1PE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5mgdA (A:)
mklssacaiallaaqaagasikhringftltehsdpakrellqkyvtwddkslfingeri
mifsgefhpfrlpvkelqldifqkvkalgfncvsfyvdwalvegkpgeyradgifdlepf
fdaaseagiyllarpgpyinaessgggfpgwlqrvngtlrssdkayldatdnyvshvaat
iakyqitnggpiilyqpeneytsgccgvefpdpvymqyvedqarnagvviplinndasas
gnnapgtgkgavdiyghdsyplgfdcanptvwpsgdlptnfrtlhleqspttpyaivqfq
ggsydpwggpgfaacsellnnefervfykndfsfqiaimnlymifggtnwgnlgypngyt
sydygsavtesrnitrekyselkllgnfakvspgyltaspgnlttsgyadttdltvtpll
gnstgsffvvrhsdysseestsyklrlptsagsvtipqlggtltlngrdskihvtdynvs
gtniiystaevftwkkfadgkvlvlyggagehhelaistksnvtviegsesgisskqtss
svvvgwdvsttrriiqvgdlkillldrnsaynywvpqlatdgtspgfstpekvassiivk
agylvrtaylkgsglyltadfnattsvevigvpstaknlfingdktshtvdkngiwsatv
dynapdislpslkdldwkyvdtlpeiqssyddslwpaadlkqtkntlrslttptslyssd
ygfhtgyllyrghftatgnestfaidtqggsafgssvwlngtylgswtglyansdynaty
nlpqlqagktyvitvvidnmgleenwtvgedlmktprgilnfllagrpssaiswkltgnl
ggedyedkvrgplnegglyaerqgfhqpeppsqnwkssspleglseagigfysasfdldl
pkgwdvplflnignsttpspyrvqvyvngyqyakyisnigpqtsfpvpegilnyrgtnwl
avtlwaldsaggkleslelsyttpvltalgevesvdqpkykkrkgayhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5mgdA (A:)
llqkyvtwddkslfingerimifsgefhpfrlpvkelqldifqkvkalgfncvsfyvdwa
lvegkpgeyradgifdlepffdaaseagiyllarpgpyinaessgggfpgwlqrvngtlr
ssdkayldatdnyvshvaatiakyqitnggpiilyqpeneytsgccgvefpdpvymqyve
dqarnagvviplinndasasgnnapgtgkgavdiyghdsyplgfdcanptvwpsgdlptn
frtlhleqspttpyaivqfqggsydpwggpgfaacsellnnefervfykndfsfqiaimn
lymifggtnwgnlgypngytsydygsavtesrnitrekyselkllgnfakvspgyltasp
gnlttsgyadttdltvtpllgnstgsffvvrhsdysseestsyklrlptsagsvtipqlg
gtltlngrdskihvtdynvsgtniiystaevftwkkfadgkvlvlyggagehhelaistk
snvtviegsesgisskqtsssvvvgwdvsttrriiqvgdlkillldrnsaynywvpqlat
dgtspgfstpekvassiivkagylvrtaylkgsglyltadfnattsvevigvpstaknlf
ingdktshtvdkngiwsatvdynapdislpslkdldwkyvdtlpeiqssyddslwpaadl
kqtkntlrslttptslyssdygfhtgyllyrghftatgnestfaidtqggsafgssvwln
gtylgswtglyansdynatynlpqlqagktyvitvvidnmgleenwtvgedlmktprgil
nfllagrpssaiswkltgnlggedyedkvrgplnegglyaerqgfhqpeppsqnwksssp
leglseagigfysasfdldlpkgwdvplflnignsttpspyrvqvyvngyqyakyisnig
pqtsfpvpegilnyrgtnwlavtlwaldsaggkleslelsyttpvltalgevesvdqpky
kkrkgayh