PDB entry 5m6x

View 5m6x on RCSB PDB site
Description: Crystal Structure of human RhoGAP mutated in its arginine finger (R85A) in complex with RhoA.GDP.MgF3- human
Class: signaling protein
Keywords: RHOGAP, Arginine finger, RHOA, transition state, Signaling protein
Deposited on 2016-10-26, released 2017-05-17
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-09-06, with a file datestamp of 2017-09-01.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: N/A
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Rho GTPase-activating protein 1
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGAP1, CDC42GAP, RHOGAP1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q07960
      • engineered mutation (84)
    Domains in SCOPe 2.07: d5m6xa_
  • Chain 'B':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARH12, ARHA, RHO12
    Database cross-references and differences (RAF-indexed):
    • Uniprot P61586
      • conflict (23)
    Domains in SCOPe 2.07: d5m6xb_
  • Chain 'H':
    Compound: Rho GTPase-activating protein 1
    Species: Homo sapiens [TaxId:9606]
    Gene: ARHGAP1, CDC42GAP, RHOGAP1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q07960
      • engineered mutation (84)
    Domains in SCOPe 2.07: d5m6xh_
  • Chain 'I':
    Compound: transforming protein rhoa
    Species: Homo sapiens [TaxId:9606]
    Gene: RHOA, ARH12, ARHA, RHO12
    Database cross-references and differences (RAF-indexed):
    • Uniprot P61586
      • conflict (23)
    Domains in SCOPe 2.07: d5m6xi_
  • Heterogens: MG, GDP, MGF, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >5m6xA (A:)
    hvkleqlgiprqvlkyddflkstqkspatapkpmpprpplpnqqfgvslqhlqeknpeqe
    pipivlretvaylqahalttegifarsantqvvrevqqkynmglpvdfdqynelhlpavi
    lktflrelpeplltfdlyphvvgflnidesqrvpatlqvlqtlpeenyqvlrfltaflvq
    isahsdqnkmtntnlavvfgpnllwakdaaitlkainpintftkflldhqgelfpspdps
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m6xA (A:)
    qqfgvslqhlqeknpipivlretvaylqahalttegifarsantqvvrevqqkynmglpv
    dfdqynelhlpavilktflrelpeplltfdlyphvvgflnidesqrvpatlqvlqtlpee
    nyqvlrfltaflvqisahsdqnkmtntnlavvfgpnllwakdaaitlkainpintftkfl
    ldhqgelf
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >5m6xB (B:)
    aairkklvivgdgacgktcllivnskdqfpevyvptvfenyvadievdgkqvelalwdta
    gqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdl
    rndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa
    rrgkkksgclvl
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m6xB (B:)
    airkklvivgdgacgktcllivnskdqfpevyvptvfenyvadievdgkqvelalwdtag
    qedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdlr
    ndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalq
    

  • Chain 'H':
    Sequence, based on SEQRES records: (download)
    >5m6xH (H:)
    hvkleqlgiprqvlkyddflkstqkspatapkpmpprpplpnqqfgvslqhlqeknpeqe
    pipivlretvaylqahalttegifarsantqvvrevqqkynmglpvdfdqynelhlpavi
    lktflrelpeplltfdlyphvvgflnidesqrvpatlqvlqtlpeenyqvlrfltaflvq
    isahsdqnkmtntnlavvfgpnllwakdaaitlkainpintftkflldhqgelfpspdps
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m6xH (H:)
    qqfgvslqhlqekpipivlretvaylqahalttegifarsantqvvrevqqkynmglpvd
    fdqynelhlpavilktflrelpeplltfdlyphvvgflnidesqrvpatlqvlqtlpeen
    yqvlrfltaflvqisahsdqnkmtntnlavvfgpnllwakdaaitlkainpintftkfll
    dhqgelf
    

  • Chain 'I':
    Sequence, based on SEQRES records: (download)
    >5m6xI (I:)
    aairkklvivgdgacgktcllivnskdqfpevyvptvfenyvadievdgkqvelalwdta
    gqedydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdl
    rndehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa
    rrgkkksgclvl
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m6xI (I:)
    irkklvivgdgacgktcllivnskdqfpevyvptvfenyvadievdgkqvelalwdtagq
    edydrlrplsypdtdvilmcfsidspdslenipekwtpevkhfcpnvpiilvgnkkdlrn
    dehtrrelakmkqepvkpeegrdmanrigafgymecsaktkdgvrevfematraalqa