PDB entry 5knl
View 5knl on RCSB PDB site
Description: Crystal structure of S. pombe ubiquitin E1 (Uba1) in complex with Ubc15 and ubiquitin
Class: ligase
Keywords: UBIQUITIN, E1, E2, UBA1, UBC15, CONFORMATIONAL CHANGE, THIOESTER, ADENYLATION, THIOESTER TRANSFER (TRANSTHIOESTERIFICATION), ATP-Binding, UBIQUITIN E2 BINDING, UBIQUITINATION, LIGASE
Deposited on
2016-06-28, released
2017-02-15
The last revision prior to the SCOPe 2.06 freeze date was dated
2017-02-15, with a file datestamp of
2017-02-10.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: N/A
AEROSPACI score: 0.21
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: Ubiquitin-activating enzyme E1 1
Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) [TaxId:284812]
Gene: ptr3, SPBC1604.21c, SPBC211.09
Database cross-references and differences (RAF-indexed):
- Chain 'B':
Compound: Ubiquitin
Species: Pseudozyma antarctica [TaxId:84753]
Gene: PAN0_008c3613
Database cross-references and differences (RAF-indexed):
- Uniprot A0A081CFF0 (20-95)
- engineered mutation (25)
- engineered mutation (30)
- engineered mutation (46)
- engineered mutation (48)
- engineered mutation (52)
- engineered mutation (67)
- engineered mutation (76)
- engineered mutation (82)
Domains in SCOPe 2.06: d5knlb_ - Chain 'C':
Compound: Ubiquitin-conjugating enzyme E2 15
Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) [TaxId:284812]
Gene: ubc15, SPBC1105.09
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.06: d5knlc1, d5knlc2 - Chain 'D':
Compound: Ubiquitin-activating enzyme E1 1
Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) [TaxId:284812]
Gene: ptr3, SPBC1604.21c, SPBC211.09
Database cross-references and differences (RAF-indexed):
- Chain 'E':
Compound: Ubiquitin
Species: Pseudozyma antarctica [TaxId:84753]
Gene: PAN0_008c3613
Database cross-references and differences (RAF-indexed):
- Uniprot A0A081CFF0 (20-95)
- engineered mutation (25)
- engineered mutation (30)
- engineered mutation (46)
- engineered mutation (48)
- engineered mutation (52)
- engineered mutation (67)
- engineered mutation (76)
- engineered mutation (82)
Domains in SCOPe 2.06: d5knle_ - Chain 'F':
Compound: Ubiquitin-conjugating enzyme E2 15
Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) [TaxId:284812]
Gene: ubc15, SPBC1105.09
Database cross-references and differences (RAF-indexed):
Domains in SCOPe 2.06: d5knlf1, d5knlf2 - Heterogens: SO4, HOH
PDB Chain Sequences:
- Chain 'A':
No sequence available.
- Chain 'B':
Sequence, based on SEQRES records: (download)
>5knlB (B:)
mgsshhhhhhssglvprgshmqifvrtltgrtitlevessdtidnvrariqdregippdq
qrlifagrqledgrtladyniqrestlhlvlrlrgg
Sequence, based on observed residues (ATOM records): (download)
>5knlB (B:)
mqifvrtltgrtitlevessdtidnvrariqdregippdqqrlifagrqledgrtladyn
iqrestlhlvlrlrgg
- Chain 'C':
Sequence, based on SEQRES records: (download)
>5knlC (C:)
mpssaseqllrkqlkeiqknppqgfsvglvddksifewevmiigpedtlyeggffhatls
fpqdyplmppkmkftteiwhpnvhpngevcisilhppgddkygyedagerwlpvhspeti
lisvismlsspndespanidaakefrenpqefkkrvrrlvrrsiemilehhhhhh
Sequence, based on observed residues (ATOM records): (download)
>5knlC (C:)
mpssaseqllrkqlkeiqknppqgfsvglvddksifewevmiigpedtlyeggffhatls
fpqdyplmppkmkftteiwhpnvhpngevcisilhppgddkygyedagerwlpvhspeti
lisvismlsspndespanidaakefrenpqefkkrvrrlvrrsiemilehh
- Chain 'D':
No sequence available.
- Chain 'E':
Sequence, based on SEQRES records: (download)
>5knlE (E:)
mgsshhhhhhssglvprgshmqifvrtltgrtitlevessdtidnvrariqdregippdq
qrlifagrqledgrtladyniqrestlhlvlrlrgg
Sequence, based on observed residues (ATOM records): (download)
>5knlE (E:)
mqifvrtltgrtitlevessdtidnvrariqdregippdqqrlifagrqledgrtladyn
iqrestlhlvlrlrgg
- Chain 'F':
Sequence, based on SEQRES records: (download)
>5knlF (F:)
mpssaseqllrkqlkeiqknppqgfsvglvddksifewevmiigpedtlyeggffhatls
fpqdyplmppkmkftteiwhpnvhpngevcisilhppgddkygyedagerwlpvhspeti
lisvismlsspndespanidaakefrenpqefkkrvrrlvrrsiemilehhhhhh
Sequence, based on observed residues (ATOM records): (download)
>5knlF (F:)
mpssaseqllrkqlkeiqknppqgfsvglvddksifewevmiigpedtlyeggffhatls
fpqdyplmppkmkftteiwhpnvhpngevcisilhppgderwlpvhspetilisvismls
spndespanidaakefrenpqefkkrvrrlvrrsiemilehh