PDB entry 5iq8

View 5iq8 on RCSB PDB site
Description: Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide
Class: hydrolase
Keywords: Enzyme, hydrolase, disulfide
Deposited on 2016-03-10, released 2017-06-28
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-12-25, with a file datestamp of 2019-12-20.
Experiment type: XRAY
Resolution: 2.06 Å
R-factor: N/A
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Beta-lactamase TEM
    Species: Escherichia coli [TaxId:562]
    Gene: bla, blaT-3, blaT-4, blaT-5, blaT-6
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62593 (0-262)
      • engineered mutation (156)
      • engineered mutation (198)
      • engineered mutation (255)
    Domains in SCOPe 2.08: d5iq8a_
  • Chain 'B':
    Compound: Beta-lactamase TEM
    Species: Escherichia coli [TaxId:562]
    Gene: bla, blaT-3, blaT-4, blaT-5, blaT-6
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62593 (0-262)
      • engineered mutation (156)
      • engineered mutation (198)
      • engineered mutation (255)
    Domains in SCOPe 2.08: d5iq8b_
  • Chain 'C':
    Compound: Beta-lactamase TEM
    Species: Escherichia coli [TaxId:562]
    Gene: bla, blaT-3, blaT-4, blaT-5, blaT-6
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62593 (0-262)
      • engineered mutation (156)
      • engineered mutation (198)
      • engineered mutation (255)
    Domains in SCOPe 2.08: d5iq8c_
  • Chain 'D':
    Compound: Beta-lactamase TEM
    Species: Escherichia coli [TaxId:562]
    Gene: bla, blaT-3, blaT-4, blaT-5, blaT-6
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62593 (0-262)
      • engineered mutation (156)
      • engineered mutation (198)
      • engineered mutation (255)
    Domains in SCOPe 2.08: d5iq8d_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5iq8A (A:)
hpetlvkvkdaedqlgarvgyieldlnsgkilesfrpeerfpmmstfkvllcgavlsrvd
agqeqlgrrihysqndlveyspvtekhltdgmtvrelcsaaitmsdntaanlllttiggp
keltaflhnmgdhvtrldrwepelneaipnderdtttpaamattlrklltgelltlasrq
qlidwmeadkvagpllrsclpagwfiadksgagergsrgiiaalgpdgkpsrivviyttg
sqatmdernrqiaeicaslikhw

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5iq8B (B:)
hpetlvkvkdaedqlgarvgyieldlnsgkilesfrpeerfpmmstfkvllcgavlsrvd
agqeqlgrrihysqndlveyspvtekhltdgmtvrelcsaaitmsdntaanlllttiggp
keltaflhnmgdhvtrldrwepelneaipnderdtttpaamattlrklltgelltlasrq
qlidwmeadkvagpllrsclpagwfiadksgagergsrgiiaalgpdgkpsrivviyttg
sqatmdernrqiaeicaslikhw

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5iq8C (C:)
hpetlvkvkdaedqlgarvgyieldlnsgkilesfrpeerfpmmstfkvllcgavlsrvd
agqeqlgrrihysqndlveyspvtekhltdgmtvrelcsaaitmsdntaanlllttiggp
keltaflhnmgdhvtrldrwepelneaipnderdtttpaamattlrklltgelltlasrq
qlidwmeadkvagpllrsclpagwfiadksgagergsrgiiaalgpdgkpsrivviyttg
sqatmdernrqiaeicaslikhw

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5iq8D (D:)
hpetlvkvkdaedqlgarvgyieldlnsgkilesfrpeerfpmmstfkvllcgavlsrvd
agqeqlgrrihysqndlveyspvtekhltdgmtvrelcsaaitmsdntaanlllttiggp
keltaflhnmgdhvtrldrwepelneaipnderdtttpaamattlrklltgelltlasrq
qlidwmeadkvagpllrsclpagwfiadksgagergsrgiiaalgpdgkpsrivviyttg
sqatmdernrqiaeicaslikhw