PDB entry 5iq0

View 5iq0 on RCSB PDB site
Description: Crystal structure of Esterase mutant - F72G
Class: hydrolase
Keywords: Esterase, Est25, Hormone-Sensitive Lipase, HYDROLASE
Deposited on 2016-03-10, released 2017-03-22
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-08-30, with a file datestamp of 2017-08-25.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: esterase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4TZQ3 (0-361)
      • engineered mutation (71)
    Domains in SCOPe 2.07: d5iq0a_
  • Chain 'B':
    Compound: esterase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4TZQ3 (Start-361)
      • engineered mutation (71)
    Domains in SCOPe 2.07: d5iq0b_
  • Chain 'C':
    Compound: esterase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4TZQ3 (Start-361)
      • engineered mutation (71)
    Domains in SCOPe 2.07: d5iq0c_
  • Chain 'D':
    Compound: esterase
    Species: Uncultured bacterium [TaxId:77133]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4TZQ3 (Start-361)
      • engineered mutation (71)
    Domains in SCOPe 2.07: d5iq0d_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download)
    >5iq0A (A:)
    msnatlnqlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciay
    staaeqawqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcv
    vhthgggmviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaa
    kwvasnrealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyask
    peelpsllendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvn
    eldplrdeglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafays
    ra
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >5iq0B (B:)
    msnatlnqlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciay
    staaeqawqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcv
    vhthgggmviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaa
    kwvasnrealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyask
    peelpsllendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvn
    eldplrdeglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafays
    ra
    

    Sequence, based on observed residues (ATOM records): (download)
    >5iq0B (B:)
    qlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciaystaaeqa
    wqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcvvhthggg
    mviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaakwvasnr
    ealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyaskpeelpsl
    lendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvneldplrd
    eglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafaysra
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >5iq0C (C:)
    msnatlnqlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciay
    staaeqawqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcv
    vhthgggmviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaa
    kwvasnrealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyask
    peelpsllendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvn
    eldplrdeglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafays
    ra
    

    Sequence, based on observed residues (ATOM records): (download)
    >5iq0C (C:)
    lpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciaystaaeqaw
    qtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcvvhthgggm
    viltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaakwvasnre
    algistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyaskpeelpsll
    endayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvneldplrde
    glahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafaysra
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >5iq0D (D:)
    msnatlnqlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciay
    staaeqawqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcv
    vhthgggmviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaa
    kwvasnrealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyask
    peelpsllendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvn
    eldplrdeglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafays
    ra
    

    Sequence, based on observed residues (ATOM records): (download)
    >5iq0D (D:)
    qlpgrlgdpsmslgtdprtdprlaaaltqlgladqaaeppvnansevadciaystaaeqa
    wqtlgamlgsqgepsnpvdvreetikgrggneiklyihsptghtsdsdplpcvvhthggg
    mviltaadanysrwrselaatglvvvgvefrnaagalgnhpfpaglhdcadaakwvasnr
    ealgistlimsgesgggnlslattmlakkegwleeiagvyaqcpyisglyaskpeelpsl
    lendayfldmktmgamvkpydptgenasnplawpyhasledlaglpphvisvneldplrd
    eglahyrkllkagvstvgrtvhgtchaadcsfvdvipdvyfatvrdisafaysra