PDB entry 5gnm

View 5gnm on RCSB PDB site
Description: Cytochrome P450 Vdh (CYP107BR1) L348M mutant
Class: oxidoreductase
Keywords: OXIDOREDUCTASE, cytochrome P450
Deposited on 2016-07-22, released 2017-05-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-09-27, with a file datestamp of 2017-09-22.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.17 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Vitamin D(3) 25-hydroxylase
    Species: Pseudonocardia autotrophica [TaxId:2074]
    Gene: vdh
    Database cross-references and differences (RAF-indexed):
    • Uniprot C4B644
      • engineered mutation (347)
    Domains in SCOPe 2.08: d5gnma_
  • Chain 'B':
    Compound: Vitamin D(3) 25-hydroxylase
    Species: Pseudonocardia autotrophica [TaxId:2074]
    Gene: vdh
    Database cross-references and differences (RAF-indexed):
    • Uniprot C4B644
      • engineered mutation (347)
    Domains in SCOPe 2.08: d5gnmb_
  • Chain 'C':
    Compound: Vitamin D(3) 25-hydroxylase
    Species: Pseudonocardia autotrophica [TaxId:2074]
    Gene: vdh
    Database cross-references and differences (RAF-indexed):
    • Uniprot C4B644 (Start-402)
      • engineered mutation (347)
    Domains in SCOPe 2.08: d5gnmc_
  • Chain 'D':
    Compound: Vitamin D(3) 25-hydroxylase
    Species: Pseudonocardia autotrophica [TaxId:2074]
    Gene: vdh
    Database cross-references and differences (RAF-indexed):
    • Uniprot C4B644 (Start-402)
      • engineered mutation (347)
    Domains in SCOPe 2.08: d5gnmd_
  • Heterogens: HEM, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5gnmA (A:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrva
igrlfadrpelalavgldelvyrestlvrglsrmpvtmgprsalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5gnmA (A:)
ltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvdpr
lskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmnelepriteia
dgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddkna
amgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghettv
nligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgvti
pagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrvaig
rlfadrpelalavgldelvyrestlvrglsrmpvtmgpr

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5gnmB (B:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrva
igrlfadrpelalavgldelvyrestlvrglsrmpvtmgprsalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5gnmB (B:)
ltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvdpr
lskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmnelepriteia
dgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddkna
amgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghettv
nligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgvti
pagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrvaig
rlfadrpelalavgldelvyrestlvrglsrmpvtmgpr

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5gnmC (C:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrva
igrlfadrpelalavgldelvyrestlvrglsrmpvtmgprsalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5gnmC (C:)
ltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvdpr
lskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmnelepriteia
dgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddkna
amgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghettv
nligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgvti
pagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrvaig
rlfadrpelalavgldelvyrestlvrglsrmpvtmgprsa

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5gnmD (D:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrva
igrlfadrpelalavgldelvyrestlvrglsrmpvtmgprsalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5gnmD (D:)
ltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvdpr
lskdwrhtlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmnelepriteia
dgllaglptdgpvdlmreyafqipvqvicellgvpaedrddfsawssvlvddspaddkna
amgklhgylsdllerkrtepddallssllavsdedgdrlsqeelvamamllliaghettv
nligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgvti
pagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfcmgaqlarlegrvaig
rlfadrpelalavgldelvyrestlvrglsrmpvtmgprsa