PDB entry 5fav

View 5fav on RCSB PDB site
Description: E491Q mutant of choline TMA-lyase
Class: lyase
Keywords: mutant, radical, lyase
Deposited on 2015-12-12, released 2016-09-28
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-27, with a file datestamp of 2019-11-22.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Choline trimethylamine-lyase
    Species: Desulfovibrio alaskensis [TaxId:207559]
    Gene: Dde_3282
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q30W70 (21-814)
      • expression tag (12-20)
      • engineered mutation (459)
    Domains in SCOPe 2.08: d5fava1, d5fava2
  • Chain 'B':
    Compound: Choline trimethylamine-lyase
    Species: Desulfovibrio alaskensis [TaxId:207559]
    Gene: Dde_3282
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q30W70 (21-814)
      • expression tag (9-20)
      • engineered mutation (459)
    Domains in SCOPe 2.08: d5favb1, d5favb2
  • Heterogens: NA, MLI, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5favA (A:)
mgsshhhhhhssglvprgshmgipdgptprhvklkenflkqvpsitvqravaitkiaken
pglpkpllraktfryccetaplviqdhelivgspngapragafspevawrwlqdeldtig
srpqdpfyiseedkkvlreevfpfwqnksvdefcegqyreadlwemsgesfvsdcsyhav
ngggdsnpgydvilmkkgmldiqrearekleqldyanpedidkiyfyksvietaegvmiy
arrlsayaaelaaretdprrkaelqkisevnarvpahapsnfweaiqavwtvesllvvee
nqtgmsigrvdqymypfyradidsgrlteyeafdlagcmlvkmsemmwitsegaskffag
yqpfvnmcvggvtreghdatndltymlmdavrhvriyqptlatrvhnkspqkylkkivdv
irsgmgfpavhfddahikmmlakgvsiedardyclmgcvqpqksgrlyqwtstgytqwpi
cielvlnhgvplwygkkvtpdmgdlsqydtyekfeaavkeqirwitkntsvatvisqrah
relapkplmslmyegcmesgrdvsaggamynfgpgvvwsglatyvdsmaaikklvyddrk
ytlaqlnealkadfagydqiladclaapkygndddyadmiaadlvhftetehrkyktlys
vlshgtlsisnntpfgqllgasangrrawmplsdgisptqgadykgptaiiksvskmand
nmnigmvhnfklmsglldtpegenglitlirtacmlgngemqfnyldnellldaqkhpek
yrdlvvrvagysaffvelckdvqdeiisrtmlhgf

    Sequence, based on observed residues (ATOM records): (download) 
    >5favA (A:)
glvprgshmgipdgptprhvklkenflkqvpsitvqravaitkiakenpglpkpllrakt
fryccetaplviqdhelivgspngapragafspevawrwlqdeldtigsrpqdpfyisee
dkkvlreevfpfwqnksvdefcegqyreadlwemsgesfvsdcsyhavngggdsnpgydv
ilmkkgmldiqrearekleqldyanpedidkiyfyksvietaegvmiyarrlsayaaela
aretdprrkaelqkisevnarvpahapsnfweaiqavwtvesllvveenqtgmsigrvdq
ymypfyradidsgrlteyeafdlagcmlvkmsemmwitsegaskffagyqpfvnmcvggv
treghdatndltymlmdavrhvriyqptlatrvhnkspqkylkkivdvirsgmgfpavhf
ddahikmmlakgvsiedardyclmgcvqpqksgrlyqwtstgytqwpicielvlnhgvpl
wygkkvtpdmgdlsqydtyekfeaavkeqirwitkntsvatvisqrahrelapkplmslm
yegcmesgrdvsaggamynfgpgvvwsglatyvdsmaaikklvyddrkytlaqlnealka
dfagydqiladclaapkygndddyadmiaadlvhftetehrkyktlysvlshgtlsisnn
tpfgqllgasangrrawmplsdgisptqgadykgptaiiksvskmandnmnigmvhnfkl
msglldtpegenglitlirtacmlgngemqfnyldnellldaqkhpekyrdlvvrvagys
affvelckdvqdeiisrtmlhgf

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5favB (B:)
mgsshhhhhhssglvprgshmgipdgptprhvklkenflkqvpsitvqravaitkiaken
pglpkpllraktfryccetaplviqdhelivgspngapragafspevawrwlqdeldtig
srpqdpfyiseedkkvlreevfpfwqnksvdefcegqyreadlwemsgesfvsdcsyhav
ngggdsnpgydvilmkkgmldiqrearekleqldyanpedidkiyfyksvietaegvmiy
arrlsayaaelaaretdprrkaelqkisevnarvpahapsnfweaiqavwtvesllvvee
nqtgmsigrvdqymypfyradidsgrlteyeafdlagcmlvkmsemmwitsegaskffag
yqpfvnmcvggvtreghdatndltymlmdavrhvriyqptlatrvhnkspqkylkkivdv
irsgmgfpavhfddahikmmlakgvsiedardyclmgcvqpqksgrlyqwtstgytqwpi
cielvlnhgvplwygkkvtpdmgdlsqydtyekfeaavkeqirwitkntsvatvisqrah
relapkplmslmyegcmesgrdvsaggamynfgpgvvwsglatyvdsmaaikklvyddrk
ytlaqlnealkadfagydqiladclaapkygndddyadmiaadlvhftetehrkyktlys
vlshgtlsisnntpfgqllgasangrrawmplsdgisptqgadykgptaiiksvskmand
nmnigmvhnfklmsglldtpegenglitlirtacmlgngemqfnyldnellldaqkhpek
yrdlvvrvagysaffvelckdvqdeiisrtmlhgf

    Sequence, based on observed residues (ATOM records): (download) 
    >5favB (B:)
hssglvprgshmgipdgptprhvklkenflkqvpsitvqravaitkiakenpglpkpllr
aktfryccetaplviqdhelivgspngapragafspevawrwlqdeldtigsrpqdpfyi
seedkkvlreevfpfwqnksvdefcegqyreadlwemsgesfvsdcsyhavngggdsnpg
ydvilmkkgmldiqrearekleqldyanpedidkiyfyksvietaegvmiyarrlsayaa
elaaretdprrkaelqkisevnarvpahapsnfweaiqavwtvesllvveenqtgmsigr
vdqymypfyradidsgrlteyeafdlagcmlvkmsemmwitsegaskffagyqpfvnmcv
ggvtreghdatndltymlmdavrhvriyqptlatrvhnkspqkylkkivdvirsgmgfpa
vhfddahikmmlakgvsiedardyclmgcvqpqksgrlyqwtstgytqwpicielvlnhg
vplwygkkvtpdmgdlsqydtyekfeaavkeqirwitkntsvatvisqrahrelapkplm
slmyegcmesgrdvsaggamynfgpgvvwsglatyvdsmaaikklvyddrkytlaqlnea
lkadfagydqiladclaapkygndddyadmiaadlvhftetehrkyktlysvlshgtlsi
snntpfgqllgasangrrawmplsdgisptqgadykgptaiiksvskmandnmnigmvhn
fklmsglldtpegenglitlirtacmlgngemqfnyldnellldaqkhpekyrdlvvrva
gysaffvelckdvqdeiisrtmlhgf