PDB entry 5ec2

View 5ec2 on RCSB PDB site
Description: KcsA with V76ester+G77dA mutations
Class: membrane protein
Keywords: alpha-helical, channel, Fab, Membrane protein
Deposited on 2015-10-20, released 2016-04-20
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-09-27, with a file datestamp of 2017-09-22.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: N/A
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: antibody fab fragment light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 5EC2 (0-218)
    Domains in SCOPe 2.07: d5ec2a1, d5ec2a2, d5ec2a3
  • Chain 'B':
    Compound: antibody fab fragment light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 5EC2 (0-211)
    Domains in SCOPe 2.07: d5ec2b1, d5ec2b2
  • Chain 'C':
    Compound: pH-gated potassium channel KcsA
    Species: Streptomyces lividans [TaxId:1916]
    Gene: kcsA, skc1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A334
      • engineered mutation (68-69)
      • engineered mutation (75-76)
      • engineered mutation (81)
    Domains in SCOPe 2.07: d5ec2c_
  • Heterogens: F09, DGA, K, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5ec2A (A:)
qvqlqqpgaelvkpgasvklsckasgytftsdwihwvkqrpghglewigeiipsygrany
nekiqkkatltadkssstafmqlssltsedsavyycarergdgyfavwgagttvtvssak
ttppsvyplapgsaaqtnsmvtlgclvkgyfpepvtvtwnsgslssgvhtfpavlqsdly
tlsssvtvpssswpsetvtcnvahpasstkvdkkivprd

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5ec2B (B:)
dilltqspailsvspgervsfscrasqsigtdihwyqqrtngsprllikyasesisgips
rfsgsgsgtdftlsinsvesedianyycqqsnrwpftfgsgtkleikradaaptvsifpp
sseqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstlt
ltkdeyerhnsytceathktstspivksfnrn

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5ec2C (C:)
mapmlsgllarlvklllgrhgsalhwraagaatvllvivllagsylavlaergapgaqli
typralwwacetattxaygdlcpvtlwgrlvavvvmvagitsfglvtaalatwfvgreqe
rrghf

    Sequence, based on observed residues (ATOM records): (download) 
    >5ec2C (C:)
alhwraagaatvllvivllagsylavlaergapgaqlitypralwwacetattxaygdlc
pvtlwgrlvavvvmvagitsfglvtaalatwfvgreqerrgh