PDB entry 5e47

View 5e47 on RCSB PDB site
Description: Synechocystis ACO mutant - T136A
Class: oxidoreductase
Keywords: Dioxygenase, Carotenoid, Cleavage, Isomerase, OXIDOREDUCTASE
Deposited on 2015-10-05, released 2015-11-04
Made obsolete by 5kk0 on 2016-08-03

The last revision prior to the SCOPe 2.06 freeze date was dated 2015-12-30, with a file datestamp of 2015-12-24.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (0-480)
      • engineered mutation (126)
    Domains in SCOPe 2.06: d5e47a_
  • Chain 'B':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (0-480)
      • engineered mutation (126)
    Domains in SCOPe 2.06: d5e47b_
  • Chain 'C':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-480)
      • engineered mutation (126)
    Domains in SCOPe 2.06: d5e47c_
  • Chain 'D':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d5e47d_
  • Heterogens: FE2, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5e47A (A:)
psqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpf
dgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlr
lkniananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpa
stfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaif
lqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanaf
eengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrcc
efpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepif
vprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaq
t

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5e47B (B:)
psqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpf
dgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlr
lkniananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpa
stfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaif
lqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanaf
eengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrcc
efpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepif
vprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaq
t

    Sequence, based on observed residues (ATOM records): (download) 
    >5e47B (B:)
psqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpf
dgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlr
lkniananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpa
stfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaif
lqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfnafeengk
iildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccefpvv
hpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvprpg
gvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5e47C (C:)
psqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpf
dgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlr
lkniananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpa
stfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaif
lqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanaf
eengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrcc
efpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepif
vprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaq
t

    Sequence, based on observed residues (ATOM records): (download) 
    >5e47C (C:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
niananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpast
fdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflq
nnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafee
ngkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccef
pvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvp
rpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5e47D (D:)
psqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpf
dgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlr
lkniananitywgdrllalweggqphrlepsnlatiglddlggilaegqplsahpridpa
stfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaif
lqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanaf
eengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrcc
efpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepif
vprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaq
t

    Sequence, based on observed residues (ATOM records): (download) 
    >5e47D (D:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
rllalweggqphrlepsnlatiglddlggilaegqplsahpridpastfdggqpcyvtfs
iksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflqnnvtlnglpylf
glrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafeengkiildsicyn
slpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccefpvvhpqqvgrpy
ryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvprpggvaeddgwl
lcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt