PDB entry 5cvj

View 5cvj on RCSB PDB site
Description: Monolignol 4-O-methyltransferase 5 - coniferyl alcohol
Class: transferase
Keywords: Monolignol 4-O-methyltransferase, lignin, S-ADENOSYL-L-HOMOCYSTEINE, coniferyl alcohol, TRANSFERASE
Deposited on 2015-07-27, released 2015-09-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-09-16, with a file datestamp of 2015-09-11.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385
      • engineered mutation (132)
      • engineered mutation (164-165)
      • engineered mutation (168)
      • engineered mutation (174)
    Domains in SCOPe 2.07: d5cvja1, d5cvja2
  • Chain 'B':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385 (Start-367)
      • engineered mutation (132)
      • engineered mutation (164-165)
      • engineered mutation (168)
      • engineered mutation (174)
    Domains in SCOPe 2.07: d5cvjb1, d5cvjb2
  • Chain 'C':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385 (Start-367)
      • engineered mutation (132)
      • engineered mutation (164-165)
      • engineered mutation (168)
      • engineered mutation (174)
    Domains in SCOPe 2.07: d5cvjc1, d5cvjc2
  • Chain 'D':
    Compound: (Iso)eugenol O-methyltransferase
    Species: Clarkia breweri [TaxId:36903]
    Gene: IEMT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot O04385 (Start-367)
      • engineered mutation (132)
      • engineered mutation (164-165)
      • engineered mutation (168)
      • engineered mutation (174)
    Domains in SCOPe 2.07: d5cvjd1, d5cvjd2
  • Heterogens: SAH, N7I, NO3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >5cvjA (A:)
    mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
    spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
    nedgvslapflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfn
    kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
    dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
    yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
    vmeflkta
    

    Sequence, based on observed residues (ATOM records): (download)
    >5cvjA (A:)
    aeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeia
    aqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvs
    lapflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfnkgmssn
    stitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafs
    gvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilpps
    pdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflk
    t
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >5cvjB (B:)
    mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
    spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
    nedgvslapflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfn
    kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
    dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
    yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
    vmeflkta
    

    Sequence, based on observed residues (ATOM records): (download)
    >5cvjB (B:)
    hssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeiaaqlpttnp
    eapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvslapfllla
    tdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfnkgmssnstitmkki
    lemyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlggd
    mfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsiatk
    vvihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkta
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >5cvjC (C:)
    mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
    spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
    nedgvslapflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfn
    kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
    dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
    yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
    vmeflkta
    

    Sequence, based on observed residues (ATOM records): (download)
    >5cvjC (C:)
    pthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeiaaqlptt
    npeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvslapfll
    latdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfnkgmssnstitmk
    kilemyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsgvehlg
    gdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppspdpsia
    tkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkta
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >5cvjD (D:)
    mgstgnaeiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyi
    spaeiaaqlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltk
    nedgvslapflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfn
    kgmssnstitmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviq
    dapafsgvehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivae
    yilppspdpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafnty
    vmeflkta
    

    Sequence, based on observed residues (ATOM records): (download)
    >5cvjD (D:)
    eiqiipthssdeeanlfamqlasaavlpmalkaaieldvleimaksvppsgyispaeiaa
    qlpttnpeapvmldrvlrllasysvvtytlrelpsgkverlyglapvckfltknedgvsl
    apflllatdkvllepwfylkdaileggipfnkaygmniwdyfgtdhrinkvfnkgmssns
    titmkkilemyngfeglttivdvgggtgavasmivakypsinainfdlphviqdapafsg
    vehlggdmfdgvpkgdaifikwichdwsdehclkllkncyaalpdhgkvivaeyilppsp
    dpsiatkvvihtdalmlaynpggkertekefqalamasgfrgfkvascafntyvmeflkt
    a