PDB entry 4wyr

View 4wyr on RCSB PDB site
Description: Crystal structure of thiolase mutation (V77Q,N153Y,A286K) from Clostridium acetobutylicum
Class: transferase
Keywords: Transferase
Deposited on 2014-11-18, released 2015-10-07
The last revision prior to the SCOPe 2.08 freeze date was dated 2015-10-14, with a file datestamp of 2015-10-09.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: N/A
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Acetyl-CoA acetyltransferase
    Species: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [TaxId:272562]
    Gene: thlA, thl, CA_C2873
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45359 (0-391)
      • engineered mutation (76)
      • engineered mutation (152)
      • engineered mutation (285)
    Domains in SCOPe 2.08: d4wyra1, d4wyra2
  • Chain 'B':
    Compound: Acetyl-CoA acetyltransferase
    Species: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [TaxId:272562]
    Gene: thlA, thl, CA_C2873
    Database cross-references and differences (RAF-indexed):
    • Uniprot P45359 (0-391)
      • engineered mutation (76)
      • engineered mutation (152)
      • engineered mutation (285)
    Domains in SCOPe 2.08: d4wyrb1, d4wyrb2
  • Heterogens: GOL, PEG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4wyrA (A:)
mkevviasavrtaigsygkslkdvpavdlgataikeavkkagikpedvnevilgnvlqag
lgqnparqasfkaglpqeipamtinkvcgsglrtvslaaqiikagdadviiaggmenmsr
apylannarwgyrmgnakfvdemitdglwdafydyhmgitaeniaerwnisreeqdefal
asqkkaeeaiksgqfkdeivpvvikgrkgetvvdtdehprfgstieglaklkpafkkdgt
vtagnasglndcaavlvimsaekakelgvkplakivsygsagvdpkimgygpfyatkaai
ekagwtvdeldliesneafaaqslavakdlkfdmnkvnvnggaialghpigasgarilvt
lvhamqkrdakkglatlcigggqgtaillekclehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4wyrA (A:)
mkevviasavrtaigsygkslkdvpavdlgataikeavkkagikpedvnevilgnvlqag
lgqnparqasfkaglpqeipamtinkvcgsglrtvslaaqiikagdadviiaggmenmsr
apylannarwgyrmgnakfvdemitdglwdafydyhmgitaeniaerwnisreeqdefal
asqkkaeeaiksgqfkdeivpvvikgrkgetvvdtdehprfgstieglaklkpafkkdgt
vtagnasglndcaavlvimsaekakelgvkplakivsygsagvdpkimgygpfyatkaai
ekagwtvdeldliesneafaaqslavakdlkfdmnkvnvnggaialghpigasgarilvt
lvhamqkrdakkglatlcigggqgtaillekc

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4wyrB (B:)
mkevviasavrtaigsygkslkdvpavdlgataikeavkkagikpedvnevilgnvlqag
lgqnparqasfkaglpqeipamtinkvcgsglrtvslaaqiikagdadviiaggmenmsr
apylannarwgyrmgnakfvdemitdglwdafydyhmgitaeniaerwnisreeqdefal
asqkkaeeaiksgqfkdeivpvvikgrkgetvvdtdehprfgstieglaklkpafkkdgt
vtagnasglndcaavlvimsaekakelgvkplakivsygsagvdpkimgygpfyatkaai
ekagwtvdeldliesneafaaqslavakdlkfdmnkvnvnggaialghpigasgarilvt
lvhamqkrdakkglatlcigggqgtaillekclehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4wyrB (B:)
mkevviasavrtaigsygkslkdvpavdlgataikeavkkagikpedvnevilgnvlqag
lgqnparqasfkaglpqeipamtinkvcgsglrtvslaaqiikagdadviiaggmenmsr
apylannarwgyrmgnakfvdemitdglwdafydyhmgitaeniaerwnisreeqdefal
asqkkaeeaiksgqfkdeivpvvikgrkgetvvdtdehprfgstieglaklkpafkkdgt
vtagnasglndcaavlvimsaekakelgvkplakivsygsagvdpkimgygpfyatkaai
ekagwtvdeldliesneafaaqslavakdlkfdmnkvnvnggaialghpigasgarilvt
lvhamqkrdakkglatlcigggqgtaillekc