PDB entry 4wxy

View 4wxy on RCSB PDB site
Description: PLPS (inactive glutaminase mutant) co-crystallized with glutamine and R5P.
Class: transferase
Keywords: beta/alpha barrel, glutamine amidotransferase, vitamin b6, pyridoxal 5-phosphate, TRANSFERASE
Deposited on 2014-11-14, released 2015-01-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-27, with a file datestamp of 2019-11-22.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2
      • conflict (225)
    Domains in SCOPe 2.08: d4wxya_
  • Chain 'B':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyb_
  • Chain 'C':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2 (10-End)
      • conflict (225)
    Domains in SCOPe 2.08: d4wxyc_
  • Chain 'D':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyd_
  • Chain 'E':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2
      • conflict (225)
    Domains in SCOPe 2.08: d4wxye_
  • Chain 'F':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyf_
  • Chain 'G':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2 (10-End)
      • conflict (225)
    Domains in SCOPe 2.08: d4wxyg_
  • Chain 'H':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyh_
  • Chain 'I':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2
      • conflict (225)
    Domains in SCOPe 2.08: d4wxyi_
  • Chain 'J':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyj_
  • Chain 'K':
    Compound: Pyridoxal biosynthesis lyase pdxS
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxS, GK0011
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y2 (10-End)
      • conflict (225)
    Domains in SCOPe 2.08: d4wxyk_
  • Chain 'L':
    Compound: Glutamine amidotransferase subunit pdxT
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: pdxT, GK0012
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5L3Y1 (32-End)
      • conflict (63)
      • engineered mutation (200)
    Domains in SCOPe 2.08: d4wxyl_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4wxyA (A:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyA (A:)
ltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvarma
dptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrqft
vpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsedel
vaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifkse
npekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4wxyB (B:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyB (B:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmqy
flnmvkea

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4wxyC (C:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyC (C:)
maltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvar
madptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrq
ftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsed
elvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifk
senpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4wxyD (D:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyD (D:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvgdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmq
yflnmvkeakma

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4wxyE (E:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyE (E:)
altgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvarm
adptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrqf
tvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsede
lvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifks
enpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >4wxyF (F:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyF (F:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvgdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmq
yflnmvkeakmass

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >4wxyG (G:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyG (G:)
maltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvar
madptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrq
ftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsed
elvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifk
senpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >4wxyH (H:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyH (H:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvgdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmq
yflnmvkeakma

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >4wxyI (I:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyI (I:)
ltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvarma
dptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrqft
vpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsedel
vaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifkse
npekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >4wxyJ (J:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyJ (J:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvgdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmq
yflnmvkea

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >4wxyK (K:)
enltpqhmasmaltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpa
diraaggvarmadptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpa
deefhidkrqftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaq
irkvvnmsedelvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgad
gvfvgsgifksenpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq
ergw

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyK (K:)
maltgtdrvkrgmaemqkggvimdvvnaeqakiaeaagavavmalervpadiraaggvar
madptvieevmnavsipvmaxvrighyvearvlealgvdyidesevltpadeefhidkrq
ftvpfvcgcrdlgeaarriaegasmlrtkgepgtgniveavrhmrkvnaqirkvvnmsed
elvaeakqlgapvevlreikrlgrlpvvnfaaggvttpadaalmmhlgadgvfvgsgifk
senpekyaraiveatthyedyeliahlskglggamrgidiatllpehrmq

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >4wxyL (L:)
mgsshhhhhhssglvprgsgtenlyfqghmasmkigvlglqgavrehvraieacgaeavi
vkkseqlegldglvlpggesttmrrlidryglmeplkqfaaagkpmfgtcaglillakri
vgydephlglmditvernsfgrqresfeaelsikgvgdgfvgvfiraphiveagdgvdvl
atyndrivaarqgqflgcsfnpeltddhrlmqyflnmvkeakmasslk

    Sequence, based on observed residues (ATOM records): (download) 
    >4wxyL (L:)
mkigvlglqgavrehvraieacgaeavivkkseqlegldglvlpggesttmrrlidrygl
meplkqfaaagkpmfgtcaglillakrivgydephlglmditvernsfgrqresfeaels
ikgvdgfvgvfiraphiveagdgvdvlatyndrivaarqgqflgcsfnpeltddhrlmqy
flnmvkeakma