PDB entry 4n56

View 4n56 on RCSB PDB site
Description: Binary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant (Cs3C KlenTaq) with DNA
Class: transferase/DNA
Keywords: DNA polymerase, dNTP, TRANSFERASE-DNA complex
Deposited on 2013-10-09, released 2015-01-21
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-02-11, with a file datestamp of 2015-02-06.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.225
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase I, thermostable
    Species: Thermus aquaticus [TaxId:271]
    Gene: polA, pol1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P19821 (Start-552)
      • engineered mutation (427)
    Domains in SCOPe 2.06: d4n56a1, d4n56a2
  • Chain 'B':
    Compound: 5'-d(*gp*ap*cp*cp*ap*cp*gp*gp*cp*gp*cp*(doc))-3'
  • Chain 'C':
    Compound: 5'-d(*ap*ap*ap*gp*gp*gp*cp*gp*cp*cp*gp*tp*gp*gp*tp*c)-3'
  • Heterogens: MN, GOL, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4n56A (A:)
mllhefgllespkaleeapwpppegafvgfvlsrkepmwadllalaaarggrvhrapepy
kalrdlkeargllakdlsvlalreglglppgddpmllaylldpsnttpegvarryggewt
eeageraalserlfanlwgrlegeerllwlyreverplsavlahmeatgvrldvaylral
slevaeeiarleaevfrlaghpfnlnsrdqlervlfdelglpaigktektgkrstsaavl
ealreahpivekilqyreltklkstyidplpdlihprtgrlhtrfnqtatatgrlsssdp
nlqnipvrtplgqrirrafiaeegwllvaldysqielrvlahlsgdenlirvfqegrdih
tetaswmfgvpreavdplmrraaktinfgvlygmsahrlsqelaipyeeaqafieryfqs
fpkvrawlektleegrrrgyvetlfgrrryvpdlearvksvreaaermafnmpvqgtaad
lmklamvklfprleemgarmllqvhdelvleapkeraeavarlakevmegvyplavplev
evgigedwlsake

    Sequence, based on observed residues (ATOM records): (download) 
    >4n56A (A:)
leeapwpppegafvgfvlsrkepmwadllalaaarggrvhrapepykalrdlkearglla
kdlsvlalreglglppgddpmllaylldpsnttpegvarryggewteeageraalserlf
anlwgrlegeerllwlyreverplsavlahmeatgvrldvaylralslevaeeiarleae
vfrlaghpfnlnsrdqlervlfdelglpaigktektgkrstsaavlealreahpivekil
qyreltklkstyidplpdlihprtgrlhtrfnqtatatgrlsssdpnlqnipvrtplgqr
irrafiaeegwllvaldysqielrvlahlsgdenlirvfqegrdihtetaaktinfgvly
gfpkvrawlektleegrrrgyvetlfgrrryvpdlearvksvreaaermafnmpvqgtaa
dlmklamvklfprleemgarmllqvhdelvleapkeraeavarlakevmegvyplavple
vevgigedwlsake

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.