PDB entry 4lnf

View 4lnf on RCSB PDB site
Description: B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of GS-Q
Class: ligase
Keywords: glutamine synthetase, GS, enzyme, alpha-beta, TnrA, GlnR-DNA, LIGASE
Deposited on 2013-07-11, released 2013-11-13
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-01-15, with a file datestamp of 2014-01-10.
Experiment type: XRAY
Resolution: 2.95 Å
R-factor: 0.195
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfa1, d4lnfa2
  • Chain 'B':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfb1, d4lnfb2
  • Chain 'C':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfc1, d4lnfc2
  • Chain 'D':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfd1, d4lnfd2
  • Chain 'E':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfe1, d4lnfe2
  • Chain 'F':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnff1, d4lnff2
  • Chain 'G':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfg1, d4lnfg2
  • Chain 'H':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfh1, d4lnfh2
  • Chain 'I':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfi1, d4lnfi2
  • Chain 'J':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfj1, d4lnfj2
  • Chain 'K':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfk1, d4lnfk2
  • Chain 'L':
    Compound: glutamine synthetase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: glnA, BSU17460
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4lnfl1, d4lnfl2
  • Heterogens: MG, GLN, PO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfA (A:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfB (B:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfC (C:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfD (D:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfE (E:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfF (F:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfG (G:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfH (H:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfI (I:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >4lnfJ (J:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

    Sequence, based on observed residues (ATOM records): (download) 
    >4lnfJ (J:)
ytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfvri
eesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemedlg
fsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgfei
eashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvngsg
mhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvpgy
eapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknkle
apapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieakei
ewdmfrtqvhpwereqymsqy

  • Chain 'K':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfK (K:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy

  • Chain 'L':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4lnfL (L:)
akytredieklvkeenvkyirlqftdilgtiknveipvsqlgkaldnkvmfdgssiegfv
rieesdmylypdlntfvifpwtaekgkvarficdiynpdgtpfegdprnnlkrilkemed
lgfsdfnlgpepefflfkldekgeptlelndkggyfdlaptdlgencrrdivleleemgf
eieashhevapgqheidfkyagavrscddiqtfklvvktiarkhglhatfmpkplfgvng
sgmhcnlslfkngvnaffdenadlqlsetakhfiagivkhatsftavtnptvnsykrlvp
gyeapcyvawsaqnrspliripasrgistrvevrsvdpaanpylalsvllaagldgiknk
leapapidrniyvmskeermengivdlpatlaealeefksnevmvkalgehlfehfieak
eiewdmfrtqvhpwereqymsqy