PDB entry 4kkl

View 4kkl on RCSB PDB site
Description: Structure of the E148A mutant of CLC-ec1 delta NC construct in 100mM fluoride
Class: tranport protein, membrane protein
Keywords: membrane transporter, tranport protein, membrane protein
Deposited on 2013-05-06, released 2013-08-21
The last revision prior to the SCOPe 2.03 freeze date was dated 2013-08-21, with a file datestamp of 2013-08-16.
Experiment type: XRAY
Resolution: 2.85 Å
R-factor: 0.251
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli [TaxId:83333]
    Gene: clcA, eriC, yadQ, b0155, JW5012
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37019 (1-444)
      • engineered mutation (132)
    Domains in SCOPe 2.03: d4kkla_
  • Chain 'B':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli [TaxId:83333]
    Gene: clcA, eriC, yadQ, b0155, JW5012
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37019
      • engineered mutation (132)
    Domains in SCOPe 2.03: d4kklb_
  • Chain 'C':
    Compound: Fab, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KKL (Start-221)
    Domains in SCOPe 2.03: d4kklc1, d4kklc2
  • Chain 'D':
    Compound: Fab, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KKL (0-210)
    Domains in SCOPe 2.03: d4kkld1, d4kkld2
  • Chain 'E':
    Compound: Fab, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KKL (Start-221)
    Domains in SCOPe 2.03: d4kkle1, d4kkle2
  • Chain 'F':
    Compound: Fab, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KKL (0-210)
    Domains in SCOPe 2.03: d4kklf1, d4kklf2
  • Heterogens: F

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4kklA (A:)
mrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadny
pllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffgg
lgtlgggmvlgragptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnapla
gilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwly
lilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsgg
gfnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmva
velfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatll
aqftggkplysailartlakqeaeqk

    Sequence, based on observed residues (ATOM records): (download) 
    >4kklA (A:)
rrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnyp
llltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffggl
gtlgggmvlgragptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplag
ilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyl
ilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsggg
fnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvav
elfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatlla
qftggkplysailartlakqeaeq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4kklB (B:)
mrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadny
pllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffgg
lgtlgggmvlgragptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnapla
gilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwly
lilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsgg
gfnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmva
velfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatll
aqftggkplysailartlakqeaeqk

    Sequence, based on observed residues (ATOM records): (download) 
    >4kklB (B:)
rrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnypl
lltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffgglg
tlgggmvlgragptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplagi
lfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyli
lgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsgggf
nlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvave
lfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatllaq
ftggkplysailartlakqea

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4kklC (C:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

    Sequence, based on observed residues (ATOM records): (download) 
    >4kklC (C:)
vrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstinyt
pslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvss
akttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqaa
lytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4kklD (D:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4kklE (E:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

    Sequence, based on observed residues (ATOM records): (download) 
    >4kklE (E:)
vrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstinyt
pslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvss
akttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqaa
lytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4kklF (F:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra