PDB entry 4ke6

View 4ke6 on RCSB PDB site
Description: Crystal structure D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in complex with 1-rac-lauroyl glycerol
Class: hydrolase
Keywords: alpha/beta hydrolase fold, Monoglyceride Lipase, Monoglycerides, HYDROLASE
Deposited on 2013-04-25, released 2013-09-18
The last revision prior to the SCOPe 2.05 freeze date was dated 2013-11-20, with a file datestamp of 2013-11-15.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.202
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597 (20-End)
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6a_
  • Chain 'B':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6b_
  • Chain 'C':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6c_
  • Chain 'D':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6d_
  • Chain 'E':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6e_
  • Chain 'F':
    Compound: Thermostable monoacylglycerol lipase
    Species: Bacillus sp. [TaxId:129908]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P82597
      • engineered mutation (215)
    Domains in SCOPe 2.05: d4ke6f_
  • Heterogens: 1QW, MPD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4ke6A (A:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6A (A:)
mseqypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgt
hyedmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpin
aavdipaiaagmggelpryldsigsdlknpdvkelayektptasllqlarlmaqtkakld
rivcpalifvsdenhvvppgnadiifqgisstekeivrlrnsyhvatldydqpmiiersl
effakha

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4ke6B (B:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6B (B:)
seqypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgth
yedmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpina
avdipaiaelpryldsigsdlknpdvkelayektptasllqlarlmaqtkakldrivcpa
lifvsdenhvvppgnadiifqgisstekeivrlrnsyhvatldydqpmiiersleffakh
a

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4ke6C (C:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6C (C:)
seqypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgth
yedmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpina
avdipaelpryldsigsdlknpdvkelayektptasllqlarlmaqtkakldrivcpali
fvsdenhvvppgnadiifqgisstekeivrlrnsyhvatldydqpmiiersleffakha

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4ke6D (D:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6D (D:)
seqypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgth
yedmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpina
avdipapryldsigsdlknpdvkelayektptasllqlarlmaqtkakldrivcpalifv
sdenhvvppgnadiifqgisstekeivrlrnsyhvatldydqpmiiersleffakha

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4ke6E (E:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6E (E:)
qypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgthye
dmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpinaav
dipadlknpdvkelayektptasllqlarlmaqtkakldrivcpalifvsdenhvvppgn
adiifqgisstekeivrlrnsyhvatldydqpmiiersleffakha

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >4ke6F (F:)
mgsshhhhhhssglvprgshmseqypvlsgaepfyaengpvgvllvhgftgtphsmrpla
eayakagytvclprlkghgthyedmerttfhdwvasveegygwlkqrcqtifvtglsmgg
tltlylaehhpdicgivpinaavdipaiaagmtgggelpryldsigsdlknpdvkelaye
ktptasllqlarlmaqtkakldrivcpalifvsdenhvvppgnadiifqgisstekeivr
lrnsyhvatldydqpmiiersleffakhag

    Sequence, based on observed residues (ATOM records): (download) 
    >4ke6F (F:)
qypvlsgaepfyaengpvgvllvhgftgtphsmrplaeayakagytvclprlkghgthye
dmerttfhdwvasveegygwlkqrcqtifvtglsmggtltlylaehhpdicgivpinaav
dipasdlknpdvkelayektptasllqlarlmaqtkakldrivcpalifvsdenhvvppg
nadiifqgisstekeivrlrnsyhvatldydqpmiiersleffakha