PDB entry 4jas
View 4jas on RCSB PDB site
Description: Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)
Class: transferase/signaling protein
Keywords: Bergerat fold, alpha/beta domain, signal transduction, autophosphorylation, phosphotransferase, dephosphorylation, histidine kinase, response regulator, phosphorylation, TRANSFERASE-SIGNALING PROTEIN complex
Deposited on
2013-02-19, released
2013-09-04
The last revision prior to the SCOPe 2.05 freeze date was dated
2013-09-04, with a file datestamp of
2013-08-30.
Experiment type: XRAY
Resolution: 3 Å
R-factor: 0.212
AEROSPACI score: 0.24
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: Histidine kinase
Species: THERMOTOGA MARITIMA [TaxId:243274]
Gene: TM_0853
Database cross-references and differences (RAF-indexed):
- Uniprot Q9WZV7
- engineered mutation (36)
- engineered mutation (39)
- engineered mutation (43)
- engineered mutation (62)
- engineered mutation (65)
Domains in SCOPe 2.05: d4jasa1, d4jasa2 - Chain 'B':
Compound: Response regulator
Species: THERMOTOGA MARITIMA [TaxId:243274]
Gene: TM_0468
Database cross-references and differences (RAF-indexed):
- Uniprot Q9WYT9 (Start-121)
- engineered mutation (12-13)
- engineered mutation (16)
- engineered mutation (20)
Domains in SCOPe 2.05: d4jasb_ - Heterogens: ADP, MG, HOH
PDB Chain Sequences:
- Chain 'A':
Sequence, based on SEQRES records: (download)
>4jasA (A:)
venvteskelerlkridrmktefianishelrtpltvikgyaemiynslgeldlstlkef
letiieqsnhlenllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnv
lfesnvpcpveayidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliived
ngigipdhakdrifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsr
ffvwipkdragednrqdn
Sequence, based on observed residues (ATOM records): (download)
>4jasA (A:)
lkridrmktefianishelrtpltvikgyaemiynslgeldlstlkefletiieqsnhle
nllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnvlfesnvpcpvea
yidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliivedngigipdhakdr
ifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsrffvwipkdr
- Chain 'B':
Sequence, based on SEQRES records: (download)
>4jasB (B:)
mskkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmd
gftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhll
ne
Sequence, based on observed residues (ATOM records): (download)
>4jasB (B:)
skkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmdg
ftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhlln
e