PDB entry 4f5l

View 4f5l on RCSB PDB site
Description: A Theoretical Optimized Mutant for the Conversion of Substrate Specificity and Activity of Aspartate Aminotransferase to Tyrosine Aminotransferase: Chimera P7.
Class: Transferase
Keywords: Aminotransferase, Transferase
Deposited on 2012-05-13, released 2013-05-15
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-05-15, with a file datestamp of 2013-05-10.
Experiment type: XRAY
Resolution: 1.4 Å
R-factor: 0.162
AEROSPACI score: 0.72 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: aspartate aminotransferase
    Species: Escherichia coli [TaxId:83333]
    Gene: AAT, aspC, b0928, JW0911
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00509 (11-405)
      • expression tag (7-10)
      • engineered mutation (38-39)
      • engineered mutation (42)
      • engineered mutation (55)
      • engineered mutation (73)
      • engineered mutation (77)
      • engineered mutation (80)
      • engineered mutation (113)
      • engineered mutation (138)
      • engineered mutation (144-145)
      • engineered mutation (196)
      • engineered mutation (219)
      • engineered mutation (221)
      • engineered mutation (227)
      • engineered mutation (253)
      • engineered mutation (294)
      • engineered mutation (384)
    Domains in SCOPe 2.08: d4f5la1, d4f5la2
  • Chain 'B':
    Compound: aspartate aminotransferase
    Species: Escherichia coli [TaxId:83333]
    Gene: AAT, aspC, b0928, JW0911
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00509 (11-405)
      • expression tag (7-10)
      • engineered mutation (38-39)
      • engineered mutation (42)
      • engineered mutation (55)
      • engineered mutation (73)
      • engineered mutation (77)
      • engineered mutation (80)
      • engineered mutation (113)
      • engineered mutation (138)
      • engineered mutation (144-145)
      • engineered mutation (196)
      • engineered mutation (219)
      • engineered mutation (221)
      • engineered mutation (227)
      • engineered mutation (253)
      • engineered mutation (294)
      • engineered mutation (384)
    Domains in SCOPe 2.08: d4f5lb1, d4f5lb2
  • Heterogens: EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4f5lA (A:)
mahhhhhhvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvmtsvk
kaeqyllenettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrva
adflakntsvkrvwvsnptwpnhkaifnsaglevreyayydaenhtldfdalinslneaq
agdvvlfhgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglra
faamhkelivasscsknfglynervgactlvaadsetvdrafsqmkaairanyssppahg
asvvatilsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsf
sgltkeqvlrlreefgvyavasgrinvagmtpdnmaplceaivavl

    Sequence, based on observed residues (ATOM records): (download) 
    >4f5lA (A:)
hvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvmtsvkkaeqyll
enettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrvaadflakn
tsvkrvwvsnptwpnhkaifnsaglevreyayydaenhtldfdalinslneaqagdvvlf
hgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglrafaamhke
livasscsknfglynervgactlvaadsetvdrafsqmkaairanyssppahgasvvati
lsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsfsgltkeq
vlrlreefgvyavasgrinvagmtpdnmaplceaivavl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4f5lB (B:)
mahhhhhhvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvmtsvk
kaeqyllenettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrva
adflakntsvkrvwvsnptwpnhkaifnsaglevreyayydaenhtldfdalinslneaq
agdvvlfhgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglra
faamhkelivasscsknfglynervgactlvaadsetvdrafsqmkaairanyssppahg
asvvatilsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsf
sgltkeqvlrlreefgvyavasgrinvagmtpdnmaplceaivavl

    Sequence, based on observed residues (ATOM records): (download) 
    >4f5lB (B:)
hvgtfenitaapadpilgladlfraderpgkvdlgvgvykdetgktpvmtsvkkaeqyll
enettktylgldglpefgrctqellfgkgsalindkrartaqtpggsgalrvaadflakn
tsvkrvwvsnptwpnhkaifnsaglevreyayydaenhtldfdalinslneaqagdvvlf
hgcchnptgadptleqwqtlaqlsvekgwlplidiayqgfgrgleedaeglrafaamhke
livasscsknfglynervgactlvaadsetvdrafsqmkaairanyssppahgasvvati
lsndalraiweqeltdmrqriqrmrqlfvntlqekganrdfsfiikqngmfsfsgltkeq
vlrlreefgvyavasgrinvagmtpdnmaplceaivavl