PDB entry 4dub

View 4dub on RCSB PDB site
Description: cytochrome P450 BM3h-9D7 MRI sensor bound to dopamine
Class: oxidoreductase
Keywords: cytochrome P450, MRI contrast sensor, directed evolution, OXIDOREDUCTASE
Deposited on 2012-02-21, released 2012-06-13
The last revision prior to the SCOPe 2.02 freeze date was dated 2012-08-29, with a file datestamp of 2012-08-24.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.166
AEROSPACI score: 0.58 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: cytochrome P450 BM3 variant 9D7
    Species: Bacillus megaterium [TaxId:1404]
    Gene: CYP102A1, cyp102
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779
      • engineered mutation (263)
      • engineered mutation (268)
      • engineered mutation (328)
      • engineered mutation (438)
  • Chain 'B':
    Compound: cytochrome P450 BM3 variant 9D7
    Species: Bacillus megaterium [TaxId:1404]
    Gene: CYP102A1, cyp102
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779
      • engineered mutation (263)
      • engineered mutation (268)
      • engineered mutation (328)
      • engineered mutation (438)
    Domains in SCOPe 2.02: d4dubb_
  • Heterogens: HEM, LDP, HOH

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4dubB (B:)
mtikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlik
eacdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhamm
vdiavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvr
aldeamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllthmln
gkdpetgeplddeniryqiitflaagheatsgllsfalyflvknphvlqkaaeeaarvlv
dpvpsykqvkqlkyvgmvlnealrlwptgpafslyakedtvlggeyplekgdelmvlipq
lhrdktiwgddveefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlk
hfdfedhtnyeldiketlvlkpegfvvkakskkiplggipspstlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4dubB (B:)
ikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikea
cdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhammvd
iavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvral
deamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllthmlngk
dpetgeplddeniryqiitflaagheatsgllsfalyflvknphvlqkaaeeaarvlvdp
vpsykqvkqlkyvgmvlnealrlwptgpafslyakedtvlggeyplekgdelmvlipqlh
rdktiwgddveefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlkhf
dfedhtnyeldiketlvlkpegfvvkakskkiplgg