PDB entry 3wtw

View 3wtw on RCSB PDB site
Description: Crystal structure of the complex comprised of ETS1(K167A), RUNX1, CBFBETA, and the tcralpha gene enhancer DNA
Class: transcription/DNA
Keywords: protein-DNA complex, transcription, DNA-binding, methylation, nucleus, phosphoprotein, transcription regulation, isopeptide bond, proto-oncogene, transcription-DNA complex
Deposited on 2014-04-21, released 2014-08-20
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-11-22, with a file datestamp of 2017-11-17.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: N/A
AEROSPACI score: 0.14 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: runt-related transcription factor 1
    Species: Mus musculus [TaxId:10090]
    Gene: Aml1, Cbfa2, Pebp2ab, Runx1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q03347 (0-End)
      • engineered mutation (34)
      • engineered mutation (107)
    Domains in SCOPe 2.07: d3wtwa_
  • Chain 'B':
    Compound: Core-binding factor subunit beta
    Species: Mus musculus [TaxId:10090]
    Gene: Cbfb, Pebp2b, Pebpb2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3wtwb_
  • Chain 'C':
    Compound: Protein C-ets-1
    Species: Homo sapiens [TaxId:9606]
    Gene: ETS1, EWSR2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3wtwc_
  • Chain 'D':
    Compound: DNA (5'-d(*gp*ap*ap*gp*cp*cp*ap*cp*ap*tp*cp*cp*tp*cp*t)-3')
  • Chain 'E':
    Compound: DNA (5'-d(*ap*gp*ap*gp*gp*ap*tp*gp*tp*gp*gp*cp*tp*tp*c)-3')
  • Chain 'F':
    Compound: runt-related transcription factor 1
    Species: Mus musculus [TaxId:10090]
    Gene: Aml1, Cbfa2, Pebp2ab, Runx1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q03347 (0-End)
      • engineered mutation (34)
      • engineered mutation (107)
    Domains in SCOPe 2.07: d3wtwf_
  • Chain 'G':
    Compound: Core-binding factor subunit beta
    Species: Mus musculus [TaxId:10090]
    Gene: Cbfb, Pebp2b, Pebpb2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3wtwg_
  • Chain 'H':
    Compound: Protein C-ets-1
    Species: Homo sapiens [TaxId:9606]
    Gene: ETS1, EWSR2
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: DNA (5'-d(*gp*ap*ap*gp*cp*cp*ap*cp*ap*tp*cp*cp*tp*cp*t)-3')
  • Chain 'J':
    Compound: DNA (5'-d(*ap*gp*ap*gp*gp*ap*tp*gp*tp*gp*gp*cp*tp*tp*c)-3')
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3wtwA (A:)
gelvrtdspnflcsvlpthwrcnktlpiafkvvakgdvpdgtlvtvmagndenysaelrn
ataamknqvarfndlrfvgrsgrgksftltitvftnppqvatyhraiaitvdgpreprrh
rqklddqtkpgslsfserlseleqlrrtamrvsphhpaptpnpraslnhstafnpqpqsq
mqdarqiqpsppwsydqsyqylgs

    Sequence, based on observed residues (ATOM records): (download) 
    >3wtwA (A:)
gelvrtdspnflcsvlpthwrcnktlpiafkvvakgdvpdgtlvtvmagndenysaelrn
ataamknqvarfndlrfvgrsgrgksftltitvftnppqvatyhraiaitvdgprepr

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3wtwB (B:)
mprvvpdqrskfeneeffrklsreceikytgfrdrpheerqtrfqnacrdgrseiafvat
gtnlslqffpaswqgeqrqtpsreyvdlereagkvylkapmilngvcviwkgwidlhrld
gmgclefdeeraqqedalaqqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3wtwB (B:)
prvvpdqrskfeneeffrklsreceikytgfrdrpheerqtrfqnacrdgrseiafvatg
tnlslqffpapsreyvdlereagkvylkapmilngvcviwkgwidlhrldgmgclefdee
raqqedalaq

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3wtwC (C:)
slqrvpsydsfdsedypaalpnhkpkgtfkdyvrdradlnkdkpvipaaalagytgsgpi
qlwqfllelltdkscqsfiswtgdgwefklsdpdevarrwgkrknkpkmnyeklsrglry
yydkniihktagkryvyrfvcdlqsllgytpeelhamldvkpdade

    Sequence, based on observed residues (ATOM records): (download) 
    >3wtwC (C:)
pvipaaalagytgsgpiqlwqfllelltdkscqsfiswtgdgwefklsdpdevarrwgkr
knkpkmnyeklsrglryyydkniihktagkryvyrfvcdlqsllgytpeelhamldvk

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3wtwF (F:)
gelvrtdspnflcsvlpthwrcnktlpiafkvvakgdvpdgtlvtvmagndenysaelrn
ataamknqvarfndlrfvgrsgrgksftltitvftnppqvatyhraiaitvdgpreprrh
rqklddqtkpgslsfserlseleqlrrtamrvsphhpaptpnpraslnhstafnpqpqsq
mqdarqiqpsppwsydqsyqylgs

    Sequence, based on observed residues (ATOM records): (download) 
    >3wtwF (F:)
gelvrtdspnflcsvlpthwrcnktlpiafkvvakgdvpdgtlvtvmagndenysaelrn
ataamknqvarfndlrfvgrsgrgksftltitvftnppqvatyhraiaitvdgpreprr

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3wtwG (G:)
mprvvpdqrskfeneeffrklsreceikytgfrdrpheerqtrfqnacrdgrseiafvat
gtnlslqffpaswqgeqrqtpsreyvdlereagkvylkapmilngvcviwkgwidlhrld
gmgclefdeeraqqedalaqqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3wtwG (G:)
prvvpdqrskfeneeffrklsreceikytgfrdrpheerqtrfqnacrdgrseiafvatg
tnlslqffpapsreyvdlereagkvylkapmilngvcviwkgwidlhrldgmgclefdee
raqqedala

  • Chain 'H':
    No sequence available.

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    No sequence available.