PDB entry 3v9i

View 3v9i on RCSB PDB site
Description: Crystal structure of human 1-pyrroline-5-carboxylate dehydrogenase mutant S352L
Class: oxidoreductase
Keywords: aldehyde dehydrogenase, Rossmann fold, nucleotide binding, acting on aldehyde or oxo group of donors, NAD or NADP as acceptor, mitochondria, OXIDOREDUCTASE
Deposited on 2011-12-27, released 2012-05-02
The last revision prior to the SCOPe 2.07 freeze date was dated 2012-06-27, with a file datestamp of 2012-06-22.
Experiment type: XRAY
Resolution: 2.85 Å
R-factor: 0.211
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: ALDH4, ALDH4A1, P5CDH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30038
      • engineered mutation (354)
    Domains in SCOPe 2.07: d3v9ia_
  • Chain 'B':
    Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: ALDH4, ALDH4A1, P5CDH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30038 (Start-565)
      • engineered mutation (354)
    Domains in SCOPe 2.07: d3v9ib_
  • Chain 'C':
    Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: ALDH4, ALDH4A1, P5CDH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30038
      • engineered mutation (354)
    Domains in SCOPe 2.07: d3v9ic_
  • Chain 'D':
    Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: ALDH4, ALDH4A1, P5CDH
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30038
      • engineered mutation (354)
    Domains in SCOPe 2.07: d3v9id_

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3v9iA (A:)
    mgsshhhhhhssglvprgshtgaglrwkhtsslkvanepvlaftqgsperdalqkalkdl
    kgrmeaipcvvgdeevwtsdvqyqvspfnhghkvakfcyadksllnkaieaalaarkewd
    lkpiadraqiflkaadmlsgprraeilaktmvgqgktviqaeidaaaelidffrfnakya
    velegqqpisvppstnstvyrglegfvaaispfnftaiggnlagapalmgnvvlwkpsdt
    amlasyavyrilreaglppniiqfvpadgplfgdtvtssehlcginftgsvptfkhlwkq
    vaqnldrfhtfprlagecggknfhfvhrsadvesvvsgtlrsafeyggqkcsaclrlyvp
    hslwpqikgrlleehsrikvgdpaedfgtffsavidaksfarikkwleharsspsltila
    ggkcddsvgyfvepciveskdpqepimkeeifgpvlsvyvypddkyketlqlvdsttsyg
    ltgavfsqdkdvvqeatkvlrnaagnfyindkstgsivgqqpfggarasgtndkpggphy
    ilrwtspqvikethkplgdwsyaymq
    

    Sequence, based on observed residues (ATOM records): (download)
    >3v9iA (A:)
    rwkhtsslkvanepvlaftqgsperdalqkalkdlkgrmeaipcvvgdeevwtsdvqyqv
    spfnhghkvakfcyadksllnkaieaalaarkewdlkpiadraqiflkaadmlsgprrae
    ilaktmvgqgktviqaeidaaaelidffrfnakyavelegpstnstvyrglegfvaaisp
    fnftaiggnlagapalmgnvvlwkpsdtamlasyavyrilreaglppniiqfvpadgplf
    gdtvtssehlcginftgsvptfkhlwkqvaqnldrfhtfprlagecgknfhfvhrsadve
    svvsgtlrsafeyggqkcsaclrlyvphslwpqikgrlleehsrikvgdpaedfgtffsa
    vidaksfarikkwleharsspsltilaggkcddsvgyfvepciveskdpqepimkeeifg
    pvlsvyvypddkyketlqlvdsttsygltgavfsqdkdvvqeatkvlrnaagnfyindks
    tilrwtspqvikethkplgdwsyaym
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3v9iB (B:)
    mgsshhhhhhssglvprgshtgaglrwkhtsslkvanepvlaftqgsperdalqkalkdl
    kgrmeaipcvvgdeevwtsdvqyqvspfnhghkvakfcyadksllnkaieaalaarkewd
    lkpiadraqiflkaadmlsgprraeilaktmvgqgktviqaeidaaaelidffrfnakya
    velegqqpisvppstnstvyrglegfvaaispfnftaiggnlagapalmgnvvlwkpsdt
    amlasyavyrilreaglppniiqfvpadgplfgdtvtssehlcginftgsvptfkhlwkq
    vaqnldrfhtfprlagecggknfhfvhrsadvesvvsgtlrsafeyggqkcsaclrlyvp
    hslwpqikgrlleehsrikvgdpaedfgtffsavidaksfarikkwleharsspsltila
    ggkcddsvgyfvepciveskdpqepimkeeifgpvlsvyvypddkyketlqlvdsttsyg
    ltgavfsqdkdvvqeatkvlrnaagnfyindkstgsivgqqpfggarasgtndkpggphy
    ilrwtspqvikethkplgdwsyaymq
    

    Sequence, based on observed residues (ATOM records): (download)
    >3v9iB (B:)
    rwkhtsslkvanepvlaftqgsperdalqkalkdlkgrmeaipcvvgdeevwtsdvqyqv
    spfnhghkvakfcyadksllnkaieaalaarkewdlkpiadraqiflkaadmlsgprrae
    ilaktmvgqgktviqaeidaaaelidffrfnakyavelegpstnstvyrglegfvaaisp
    fnftaiggnlagapalmgnvvlwkpsdtamlasyavyrilreaglppniiqfvpadgplf
    gdtvtssehlcginftgsvptfkhlwkqvaqnldrfhtfprlagecgknfhfvhrsadve
    svvsgtlrsafeyggqkcsaclrlyvphslwpqikgrlleehsrikvgdpaedfgtffsa
    vidaksfarikkwleharsspsltilaggkcddsvgyfvepciveskdpqeeifgpvlsv
    yvypddkyketlqlvdsttsygltgavfsqdkdvvqeatkvlrnaagnfyindkstilrw
    tspqvikethkplgdwsyaymq
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3v9iC (C:)
    mgsshhhhhhssglvprgshtgaglrwkhtsslkvanepvlaftqgsperdalqkalkdl
    kgrmeaipcvvgdeevwtsdvqyqvspfnhghkvakfcyadksllnkaieaalaarkewd
    lkpiadraqiflkaadmlsgprraeilaktmvgqgktviqaeidaaaelidffrfnakya
    velegqqpisvppstnstvyrglegfvaaispfnftaiggnlagapalmgnvvlwkpsdt
    amlasyavyrilreaglppniiqfvpadgplfgdtvtssehlcginftgsvptfkhlwkq
    vaqnldrfhtfprlagecggknfhfvhrsadvesvvsgtlrsafeyggqkcsaclrlyvp
    hslwpqikgrlleehsrikvgdpaedfgtffsavidaksfarikkwleharsspsltila
    ggkcddsvgyfvepciveskdpqepimkeeifgpvlsvyvypddkyketlqlvdsttsyg
    ltgavfsqdkdvvqeatkvlrnaagnfyindkstgsivgqqpfggarasgtndkpggphy
    ilrwtspqvikethkplgdwsyaymq
    

    Sequence, based on observed residues (ATOM records): (download)
    >3v9iC (C:)
    rwkhtsslkvanepvlaftqgsperdalqkalkdlkgrmeaipcvvgdeevwtsdvqyqv
    spfnhghkvakfcyadksllnkaieaalaarkewdlkpiadraqiflkaadmlsgprrae
    ilaktmvgqgktviqaeidaaaelidffrfnakyavelegqqpstnstvyrglegfvaai
    spfnftaiggnlagapalmgnvvlwkpsdtamlasyavyrilreaglppniiqfvpadgp
    lfgdtvtssehlcginftgsvptfkhlwkqvaqnldrfhtfprlagecgknfhfvhrsad
    vesvvsgtlrsafeyggqkcsaclrlyvphslwpqikgrlleehsrikvgdpaedfgtff
    savidaksfarikkwleharsspstilaggkcddsvgyfvepciveskdpqeeeifgpvl
    svyvypddkyketlqlvdsttsygltgavfsqdkdvvqeatkvlrnaagnfyindkstil
    rwtspqviketh
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >3v9iD (D:)
    mgsshhhhhhssglvprgshtgaglrwkhtsslkvanepvlaftqgsperdalqkalkdl
    kgrmeaipcvvgdeevwtsdvqyqvspfnhghkvakfcyadksllnkaieaalaarkewd
    lkpiadraqiflkaadmlsgprraeilaktmvgqgktviqaeidaaaelidffrfnakya
    velegqqpisvppstnstvyrglegfvaaispfnftaiggnlagapalmgnvvlwkpsdt
    amlasyavyrilreaglppniiqfvpadgplfgdtvtssehlcginftgsvptfkhlwkq
    vaqnldrfhtfprlagecggknfhfvhrsadvesvvsgtlrsafeyggqkcsaclrlyvp
    hslwpqikgrlleehsrikvgdpaedfgtffsavidaksfarikkwleharsspsltila
    ggkcddsvgyfvepciveskdpqepimkeeifgpvlsvyvypddkyketlqlvdsttsyg
    ltgavfsqdkdvvqeatkvlrnaagnfyindkstgsivgqqpfggarasgtndkpggphy
    ilrwtspqvikethkplgdwsyaymq
    

    Sequence, based on observed residues (ATOM records): (download)
    >3v9iD (D:)
    rwkhtsslkvanepvlaftqgsperdalqkalkdlgrmeaipcvvgdeevwtsdvqyqvs
    pfnhgvakfcyadkslnkaieaalaarkewdlkpiadraqiflkaadmlsgprraeilak
    tmvgqgktviqaeidaaaelidffrfnakyavelegqpstnstvyrglegfvaaispfnf
    taiggnlagapalmgnvvlwkpsdtamlasyavyrilreaglppniiqfvpadgplfgdt
    vtssehlcginftgsvptfkhlwkqvaqnldrfhtfprlagecgknfhfvhrsadvesvv
    sgtlrsafeyggqcsaclrlyvhslwpqikgrlleehrikvffsavidaksfarkwleha
    rsspsltilaggkcddsyfvepciveskdpqeeifgpvlsvyvypddkyketlqlvdstt
    sygltgavfsqdkdvvqeatkvlrnaagnfyindkstilrwtspqvikethkplgdwsya
    ym