PDB entry 3tn5

View 3tn5 on RCSB PDB site
Description: Crystal structure of GkaP mutant Y99L from Geobacillus kaustophilus HTA426
Class: hydrolase
Keywords: phosphotriesterase, lactonase, HYDROLASE
Deposited on 2011-09-01, released 2012-09-12
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-09-12, with a file datestamp of 2012-09-07.
Experiment type: XRAY
Resolution: 1.75 Å
R-factor: 0.222
AEROSPACI score: 0.5 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5 (Start-359)
      • engineered mutation (132)
    Domains in SCOPe 2.08: d3tn5a_
  • Chain 'B':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5 (Start-359)
      • engineered mutation (132)
    Domains in SCOPe 2.08: d3tn5b_
  • Heterogens: CO, PO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3tn5A (A:)
mgsshhhhhhssglvprgshmasmtggqqmgrgsmaemvetvcgpvpveqlgktlihehf
lfgypgfqgdvtrgtfredeslrvaveaaekmkrhgiqtvvdptpndcgrnpaflrrvae
etglniicatgylyegegappyfqfrrllgtaeddiydmfmaeltegiadtgikagvikl
asskgriteyekmffraaaraqketgaviithtqegtmgpeqaayllehgadpkkivigh
mcgntdpdyhrktlaygvyiafdrfgiqgmvgaptdeervrtllallrdgyekqimlshd
tvnvwlgrpftlpepfaemmknwhvehlfvniipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >3tn5A (A:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgylyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshdtvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfsa

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3tn5B (B:)
mgsshhhhhhssglvprgshmasmtggqqmgrgsmaemvetvcgpvpveqlgktlihehf
lfgypgfqgdvtrgtfredeslrvaveaaekmkrhgiqtvvdptpndcgrnpaflrrvae
etglniicatgylyegegappyfqfrrllgtaeddiydmfmaeltegiadtgikagvikl
asskgriteyekmffraaaraqketgaviithtqegtmgpeqaayllehgadpkkivigh
mcgntdpdyhrktlaygvyiafdrfgiqgmvgaptdeervrtllallrdgyekqimlshd
tvnvwlgrpftlpepfaemmknwhvehlfvniipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >3tn5B (B:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgylyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshdtvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfsa