PDB entry 3rh5

View 3rh5 on RCSB PDB site
Description: DNA Polymerase Beta Mutant (Y271) with a dideoxy-terminated primer with an incoming deoxynucleotide (dCTP)
Class: transferase/DNA
Keywords: DNA Polymerase Beta mutant, nucleotide transferase, ribonucleotide insertion, dCTP, TRANSFERASE-DNA complex
Deposited on 2011-04-11, released 2011-07-06
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-09-21, with a file datestamp of 2011-09-16.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.215
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase beta
    Species: Homo sapiens [TaxId:9606]
    Gene: POLB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P06746 (Start-334)
      • engineered mutation (270)
    Domains in SCOPe 2.08: d3rh5a1, d3rh5a2, d3rh5a3
  • Chain 'D':
    Compound: 5'-d(p*gp*tp*cp*gp*g)-3'
  • Chain 'P':
    Compound: 5'-d(*gp*cp*tp*gp*ap*tp*gp*cp*gp*(ddg))-3'
  • Chain 'T':
    Compound: 5'-d(*cp*cp*gp*ap*cp*gp*cp*cp*gp*cp*ap*tp*cp*ap*gp*c)-3'
  • Heterogens: DCP, MN, NA, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3rh5A (A:)
mskrkapqetlnggitdmltelanfeknvsqaihkynayrkaasviakyphkiksgaeak
klpgvgtkiaekideflatgklrklekirqddtsssinfltrvsgigpsaarkfvdegik
tledlrknedklnhhqriglkyfgdfekripreemlqmqdivlnevkkvdseyiatvcgs
frrgaessgdmdvllthpsftsestkqpkllhqvveqlqkvhfitdtlskgetkfmgvcq
lpskndekeyphrridirlipkdqyycgvlaftgsdifnknmrahalekgftineytirp
lgvtgvageplpvdsekdifdyiqwkyrepkdrse

    Sequence, based on observed residues (ATOM records): (download) 
    >3rh5A (A:)
tlnggitdmltelanfeknvsqaihkynayrkaasviakyphkiksgaeakklpgvgtki
aekideflatgklrklekirqddtsssinfltrvsgigpsaarkfvdegiktledlrkne
dklnhhqriglkyfgdfekripreemlqmqdivlnevkkvdseyiatvcgsfrrgaessg
dmdvllthpsftsestkqpkllhqvveqlqkvhfitdtlskgetkfmgvcqlpskndeke
yphrridirlipkdqyycgvlaftgsdifnknmrahalekgftineytirplgvtgvage
plpvdsekdifdyiqwkyrepkdrse

  • Chain 'D':
    No sequence available.

  • Chain 'P':
    No sequence available.

  • Chain 'T':
    No sequence available.