SCOPe 2.03: Structural Classification of Proteins

PDB entry 3pwg

View 3pwg on RCSB PDB site
Description: Crystal structure of the mutant S29G.P34A of D-Glucarate dehydratase from Escherichia coli complexed with product 5-keto-4-deoxy-D-Glucarate
Class: lyase
Keywords: Enolase superfamily fold, D-Glucarate dehydratase, 5-keto-4-deoxy-D-Glucarate, LYASE
Deposited on 2010-12-08, released 2011-12-28
The last revision prior to the SCOPe 2.03 freeze date was dated 2011-12-28, with a file datestamp of 2011-12-23.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.234
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: glucarate dehydratase
Species: Escherichia coli [TaxId:444449]
Gene: ECH74042_A2717
Database cross-references and differences (RAF-indexed):
- Uniprot E2KTD9 (Start-445)
  - engineered mutation (28)
  - engineered mutation (33)
Domains in SCOPe 2.03: d3pwga1, d3pwga2
Chain 'B':
Compound: glucarate dehydratase
Species: Escherichia coli [TaxId:444449]
Gene: ECH74042_A2717
Database cross-references and differences (RAF-indexed):
- Uniprot E2KTD9 (Start-445)
  - engineered mutation (28)
  - engineered mutation (33)
Domains in SCOPe 2.03: d3pwgb1, d3pwgb2
Chain 'C':
Compound: glucarate dehydratase
Species: Escherichia coli [TaxId:444449]
Gene: ECH74042_A2717
Database cross-references and differences (RAF-indexed):
- Uniprot E2KTD9 (Start-445)
  - engineered mutation (28)
  - engineered mutation (33)
Domains in SCOPe 2.03: d3pwgc1
Chain 'D':
Compound: glucarate dehydratase
Species: Escherichia coli [TaxId:444449]
Gene: ECH74042_A2717
Database cross-references and differences (RAF-indexed):
- Uniprot E2KTD9 (Start-445)
  - engineered mutation (28)
  - engineered mutation (33)
Domains in SCOPe 2.03: d3pwgd1, d3pwgd2
Heterogens: MG, GLR, GOL, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>3pwgA (A:)
mssqfttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggek
irktledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaam
ldllgqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrhe
eamtpdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngaw
slneaikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmgh
tlslqsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapg
kitaidthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglg
arddamgmqylipgwtfdnkrpcmvr

Sequence, based on observed residues (ATOM records): (download)

>3pwgA (A:)
fttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggekirkt
ledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaamldll
gqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrheeamt
pdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngawslne
aikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmghtlsl
qsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapgkita
idthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglgardd
amgmqylipgwtfdnkrpcmvr

Chain 'B':
Sequence, based on SEQRES records: (download)

>3pwgB (B:)
mssqfttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggek
irktledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaam
ldllgqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrhe
eamtpdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngaw
slneaikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmgh
tlslqsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapg
kitaidthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglg
arddamgmqylipgwtfdnkrpcmvr

Sequence, based on observed residues (ATOM records): (download)

>3pwgB (B:)
fttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggekirkt
ledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaamldll
gqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrheeamt
pdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngawslne
aikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmghtlsl
qsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapgkita
idthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglgardd
amgmqylipgwtfdnkrpcmvr

Chain 'C':
Sequence, based on SEQRES records: (download)

>3pwgC (C:)
mssqfttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggek
irktledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaam
ldllgqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrhe
eamtpdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngaw
slneaikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmgh
tlslqsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapg
kitaidthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglg
arddamgmqylipgwtfdnkrpcmvr

Sequence, based on observed residues (ATOM records): (download)

>3pwgC (C:)
qfttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggekirk
tledaiplvvgktlgeyknvltlvrntfadrdlrttihvvtgieaamldllgqhlgvnva
sllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrheeamtpdavvrlae
aayekygfndfklkggvlageeeaesivalaqrfpqaritldpngawslneaikigkylk
gslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmghtlslqsvdiplad
phfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapgkitaidthwiwqe
gnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglgarddamgmqylip
gwtfdnkrpcmvr

Chain 'D':
Sequence, based on SEQRES records: (download)

>3pwgD (D:)
mssqfttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggek
irktledaiplvvgktlgeyknvltlvrntfadrdaggrglqtfdlrttihvvtgieaam
ldllgqhlgvnvasllgdgqqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrhe
eamtpdavvrlaeaayekygfndfklkggvlageeeaesivalaqrfpqaritldpngaw
slneaikigkylkgslayaedpcgaeqgfsgrevmaefrratglptatnmiatdwrqmgh
tlslqsvdipladphfwtmqgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapg
kitaidthwiwqegnqrltkepfeikgglvqvpekpglgveidmdqvmkahelyqkhglg
arddamgmqylipgwtfdnkrpcmvr

Sequence, based on observed residues (ATOM records): (download)

>3pwgD (D:)
fttpvvtemqvipvaghdsmlmnlggahaafftrniviikdnsghtgvgeipggekirkt
ledaiplvvgktlgeyknvltlvrntfattihvvtgieaamldllgqhlgvnvasllgdg
qqrsevemlgylffvgnrkatplpyqsqpddscdwyrlrheeamtpdavvrlaeaayeky
gfndfklkggvlageeeaesivalaqrfpqaritldpngawslneaikigkylkgslaya
edpcgaeqgfsgrevmaefrratglptatnmiatdwrqmghtlslqsvdipladphfwtm
qgsvrvaqmchefgltwgshsnnhfdislamfthvaaaapgkitaidthwiwqegnqrlt
kepfeikgglvqvpekpglgveidmdqvmkahelyqkhglgarddamgmqylipgwtfdn
krpcmvr