PDB entry 3puz

View 3puz on RCSB PDB site
Description: Crystal Structure of a pre-translocation state MBP-Maltose transporter complex bound to AMP-PNP
Class: transport protein, membrane protein
Keywords: ATP Binding Cassette, Nucleotide Binding Domain, Substrate Binding Protein, ABC Transporter, importer, ATPase, ATP binding, Maltodextrin binding, transmembrane integral membrane, TRANSPORT PROTEIN, MEMBRANE PROTEIN
Deposited on 2010-12-06, released 2011-05-18
The last revision prior to the SCOPe 2.02 freeze date was dated 2011-06-22, with a file datestamp of 2011-06-17.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.239
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Fused maltose transport subunit, ATP-binding component of ABC superfamily; regulatory protein
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4224, malK
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Fused maltose transport subunit, ATP-binding component of ABC superfamily; regulatory protein
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4224, malK
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: Maltose transporter subunit; periplasmic-binding component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4223, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot B1XC33 (0-369)
      • engineered mutation (68)
      • engineered mutation (336)
    Domains in SCOPe 2.02: d3puze_
  • Chain 'F':
    Compound: Maltose transporter subunit; membrane component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4222, malF
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Maltose transporter subunit; membrane component of ABC superfamily
    Species: Escherichia coli [TaxId:83333]
    Gene: ECDH10B_4221, malG
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.02: d3puzg_
  • Heterogens: MAL, PGV, MG, ANP, HOH

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3puzE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfgcyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyeeelakdpriaatmenaqkgeimpnipqmcafwyavrtavinaasgrqtvdea
lkdaqtritk

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3puzG (G:)
mamvqpksqkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwk
lalgfsveqadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkat
llkgmlifqmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgy
fetidssleeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdv
nsytlavgmqqylnpqnylwgdfaaaavmsalpitivfllaqrwlvngltaggvkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3puzG (G:)
amvqpksqkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwkl
algfsveqadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkatl
lkgmlifqmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgyf
etidssleeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdvn
sytlavgmqqylnpqnylwgdfaaaavmsalpitivfllaqr